In vitro cytotoxic activities of Cyclamen L. (Primulaceae) ethanol extracts from Turkey

Abstract

We have evaluated the cytotoxic activities of the ethanol extracts of tubers and leaves of eight Cyclamen L. taxa (C. alpinum Dammann ex Spreng., C. cilicicum Boiss. & Heldr., C. coum Mill. subsp. coum, C. graecum subsp. anatolicum Ietsw., C. hederifolium Aiton, C. mirabile Hildebr., C. persicum Mill., and C. pseudibericum Hildebr.) collected from Turkey by using brine shrimp (Artemia salina L.) lethality test and the LC50 values of the extracts were also determined. The strongest cytotoxic activities were obtained by ethanolic tuber extracts. LC50 values were calculated by EPA Probit Analysis Program (version 1.5). The extracts of eight taxa showed moderate cytotoxicity against brine shrimp (LC50 between 2.93 and 533.22 µg/mL). The extracts of C. graecum subsp. anatolicum and C. persicum showed significant cytotoxicity with LC50 values of 2.93 and 3.60 µg/mL, respectively while the extract of C. pseudibericum had LC50 at 533.22 µg/ml. It could be seen among eight tested plant taxa that C. graecum subsp. anatolicum had the most cytotoxicity. © 2016 PSP.