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https://hdl.handle.net/11499/9933
Title: | Effects of ultrasonication on damaged spermatozoa and mitochondrial activity rate | Authors: | Peker, Pınar Akalın Başpınar, Nuri Çoyan, Kenan Bucak, Mustafa Numan Güngör, Şükrü Öztürk, Caner |
Keywords: | Damaged sperm rate Lipid peroxidation Mitochondrial activity Sonication Spermatozoa |
Publisher: | TUBITAK | Abstract: | The aim of this study was to describe an optimal sonication procedure for sperm cells. Therefore, we used several parameters such as damaged spermatozoa rate (%), mitochondrial activity rate (%), levels of lipid peroxidation, and total antioxidant potential. Ejaculates were collected from rams (n = 3) and were divided into aliquots and 3-, 6-, and 10-s duration times; 1, 3, 5, and 8 repetitive application groups were established. In the groups with 3-, 6- and 10-s duration times, with the increasing number of repeated applications, damaged spermatozoa rates increased (P < 0.05) while mitochondrial activity rates decreased (P < 0.05). In relation with sonication duration time, total antioxidant potential levels increased (P < 0.05) in single-application groups compared to those in control groups and gradually decreased as the repetitions increased. The most effective results were obtained in the group with 8 repetitions and 10-s duration based on damaged spermatozoa rate and mitochondrial activity rate. | URI: | https://hdl.handle.net/11499/9933 https://doi.org/10.3906/vet-1507-18 |
ISSN: | 1300-0128 |
Appears in Collections: | Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection Tıp Fakültesi Koleksiyonu TR Dizin İndeksli Yayınlar Koleksiyonu / TR Dizin Indexed Publications Collection WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection |
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