Please use this identifier to cite or link to this item: https://hdl.handle.net/11499/9954
Title: Investigation of the effects of a sulfite molecule on human neuroblastoma cells via a novel oncogene URG4/URGCP [Article]
Authors: Dodurga, Yavuz
Seçme, Mücahit
Eroğlu, Canan
Gündoğdu, Gülşah
Avcı, Ç.B.
Bağcı, Gülseren
Küçükatay, Vural
Keywords: Neuroblastoma
SH-SY5Y cells
Sulfite
URG4/URGCP
antineoplastic agent
caspase 2
caspase 3
caspase 9
complementary DNA
cyclin D1
cyclin D2
cyclin dependent kinase 4
cyclin dependent kinase 6
protein Bax
protein bcl 2
protein Bid
protein p53
retinoblastoma protein
retinoblastoma protein 1
second mitochondrial activator of caspase
sulfite
transcription factor E2F4
unclassified drug
tumor protein
URG4 protein, human
antineoplastic activity
antiproliferative activity
apoptosis
Article
cancer inhibition
cell count
cell cycle arrest
cell cycle G1 phase
cell cycle regulation
cell invasion
cell migration
cell shape
cell size
cell viability
colony formation
concentration response
controlled study
cytotoxicity
DNA damage
DNA synthesis
down regulation
gene expression
genetic analysis
genotoxicity
growth inhibition
human
human cell
IC50
in vitro study
metastasis
neuroblastoma cell line
oncogene
oncogene URG4
protein expression
tumor invasion
upregulation
antagonists and inhibitors
biosynthesis
cell survival
dose response
drug effects
metabolism
neuroblastoma
physiology
treatment outcome
tumor cell line
Cell Line, Tumor
Cell Survival
Dose-Response Relationship, Drug
Humans
Neoplasm Proteins
Oncogenes
Sulfites
Treatment Outcome
Publisher: Elsevier Inc.
Abstract: Aim: The aimof this study is to determine the anticancer effect of sulfite on SH-SY5Y neuroblastoma cells in vitro conditions and elucidate underlying molecular mechanism of sulfite and explore its therapeutic activity. Main methods: In this study, cytotoxic effects of sulfite in SH-SY5Y cellswere detected over time in a dose dependent manner with the IC50 doses ranging from 0.5 to10 mM. Genotoxic effect of sulfite was shown by comet assay. IC50 doses in the SH-SY5Y cells were detected as 5 mM. Expression profiles of the target genes related to apoptosis and cell cycle control were determined by quantitative RT-PCR. Protein changes were determined by western blot analysis. Key findings: URG4/URGCP, CCND1, CCND2, CDK4, CDK6, E2F4 and BCL-2 gene expression levels were significantly reduced and RB1, TP53, BAX, BID, CASP2, CASP3, CASP9 and DIABLO gene expressions were significantly increased in dose group cells. The mechanism of this result may be related to sulfite dependent inhibition of cell cycle at the G1 phase by down-regulating URG4/URGCP or CCND1, CDK4, CDK6 gene expression and stimulating apoptosis via the intrinsic pathway. Sulfite suppressed invasion and colony formation in SH-SY5Y cell line using matrigel invasion chamber and colony formation assay, respectively. Significance: It is thought that sulfite demonstrates anticarcinogenesis activity by affecting cell cycle arrest, apoptosis, invasion, and colony formation on SH-SY5Y cells. Sulfite may be an effective agent for treatment of neuroblastoma as a single agent or in combination with other agents. © 2015 Elsevier Inc. All rights reserved.
URI: https://hdl.handle.net/11499/9954
https://doi.org/10.1016/j.lfs.2015.10.005
ISSN: 0024-3205
Appears in Collections:PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
Tıp Fakültesi Koleksiyonu
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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