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https://hdl.handle.net/11499/9954
Title: | Investigation of the effects of a sulfite molecule on human neuroblastoma cells via a novel oncogene URG4/URGCP [Article] | Authors: | Dodurga, Yavuz Seçme, Mücahit Eroğlu, Canan Gündoğdu, Gülşah Avcı, Ç.B. Bağcı, Gülseren Küçükatay, Vural |
Keywords: | Neuroblastoma SH-SY5Y cells Sulfite URG4/URGCP antineoplastic agent caspase 2 caspase 3 caspase 9 complementary DNA cyclin D1 cyclin D2 cyclin dependent kinase 4 cyclin dependent kinase 6 protein Bax protein bcl 2 protein Bid protein p53 retinoblastoma protein retinoblastoma protein 1 second mitochondrial activator of caspase sulfite transcription factor E2F4 unclassified drug tumor protein URG4 protein, human antineoplastic activity antiproliferative activity apoptosis Article cancer inhibition cell count cell cycle arrest cell cycle G1 phase cell cycle regulation cell invasion cell migration cell shape cell size cell viability colony formation concentration response controlled study cytotoxicity DNA damage DNA synthesis down regulation gene expression genetic analysis genotoxicity growth inhibition human human cell IC50 in vitro study metastasis neuroblastoma cell line oncogene oncogene URG4 protein expression tumor invasion upregulation antagonists and inhibitors biosynthesis cell survival dose response drug effects metabolism neuroblastoma physiology treatment outcome tumor cell line Cell Line, Tumor Cell Survival Dose-Response Relationship, Drug Humans Neoplasm Proteins Oncogenes Sulfites Treatment Outcome |
Publisher: | Elsevier Inc. | Abstract: | Aim: The aimof this study is to determine the anticancer effect of sulfite on SH-SY5Y neuroblastoma cells in vitro conditions and elucidate underlying molecular mechanism of sulfite and explore its therapeutic activity. Main methods: In this study, cytotoxic effects of sulfite in SH-SY5Y cellswere detected over time in a dose dependent manner with the IC50 doses ranging from 0.5 to10 mM. Genotoxic effect of sulfite was shown by comet assay. IC50 doses in the SH-SY5Y cells were detected as 5 mM. Expression profiles of the target genes related to apoptosis and cell cycle control were determined by quantitative RT-PCR. Protein changes were determined by western blot analysis. Key findings: URG4/URGCP, CCND1, CCND2, CDK4, CDK6, E2F4 and BCL-2 gene expression levels were significantly reduced and RB1, TP53, BAX, BID, CASP2, CASP3, CASP9 and DIABLO gene expressions were significantly increased in dose group cells. The mechanism of this result may be related to sulfite dependent inhibition of cell cycle at the G1 phase by down-regulating URG4/URGCP or CCND1, CDK4, CDK6 gene expression and stimulating apoptosis via the intrinsic pathway. Sulfite suppressed invasion and colony formation in SH-SY5Y cell line using matrigel invasion chamber and colony formation assay, respectively. Significance: It is thought that sulfite demonstrates anticarcinogenesis activity by affecting cell cycle arrest, apoptosis, invasion, and colony formation on SH-SY5Y cells. Sulfite may be an effective agent for treatment of neuroblastoma as a single agent or in combination with other agents. © 2015 Elsevier Inc. All rights reserved. | URI: | https://hdl.handle.net/11499/9954 https://doi.org/10.1016/j.lfs.2015.10.005 |
ISSN: | 0024-3205 |
Appears in Collections: | PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection Tıp Fakültesi Koleksiyonu WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection |
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