Acıpayam Meslek Yüksekokulu Koleksiyonu
Permanent URI for this collectionhttps://hdl.handle.net/11499/46010
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Article Citation - WoS: 1Citation - Scopus: 1Effects of Vitamin C Supplementation on Total Antioxidant Status, Inflammation, and Histopathological Changes in Aged Rats(John Wiley and Sons Inc, 2025-05-30) Basegmez, Mehmet; Eryavuz, Abdullah; Demirel, Hasan HuseyinThis study aims to determine the effect of orally administered vitamin C (Vit C) supplementation on physiological and histopathological changes in aged rats of different genders. A total of 28 Sprague-Dawley aged male and female rats were randomly divided into four groups of seven animals per group. The study groups included the aged male control (MC), aged male with Vit C (MVC) (500 mg/kg vitamin C, orally) supplementation, female aged control (FC), and female aged with vitamin C (FVC) (500 mg/kg vitamin C, orally) supplementation groups. At the end of the study, which lasted 31 days, blood, brain, heart, liver, and kidney tissues were collected from rats under ketamine (87 mg/kg) and xylazine (13 mg/kg) anesthesia. The results indicated that although Vit C supplementation had no effect on serum Vit C levels, gender had an effect on serum Vit C levels (p < 0.05). However, Vit C supplementation and gender did not affect serum IL-6, IL-1β, TOS, and OSI levels (p > 0.05). Vit C supplementation, without the effect of gender, significantly increased TNF-α levels in MVC groups compared to MC groups (p < 0.05), while it significantly decreased them in FVC groups compared to FC groups (p < 0.05). In addition, Vit C significantly reduced histopathological alterations in brain, heart, and liver tissues associated with aging, including oxidative stress and inflammation. In conclusion, it was observed that orally administered 500 mg/kg Vit C supplementation to old rats is not an effective way to increase the Vit C pool in the body, but gender has an impact on the blood Vit C concentrations. © 2025 The Author(s). Journal of Biochemical and Molecular Toxicology published by Wiley Periodicals LLC.Article Citation - WoS: 2Citation - Scopus: 3The effect of boric acid on oxidative stress, inflammation, and apoptosis in embryonic and fetal tissues damage caused by consumption of high-fructose corn syrup in pregnant rats(Springer Heidelberg, 2025-01-16) Basegmez, Mehmet; Yuksel, DuyguThis study aimed to determine the protective role of boric acid in a pregnant rat model of high fructose corn syrup consumption. Consumption of high fructose corn syrup has been associated with adverse health outcomes in humans and animals. Twenty-eight healthy female Wistar albino rats (250-300 g weight and 16-24 weeks old) were randomly distributed into four equal groups (n = 7): Control, Boric acid (BA), High Fructose Corn Syrup (HFCS), HFCS + BA. Boric acid (20 mg/kg) was administered to pregnant rats via oral gavage every day during pregnancy. The prepared 30% HFCS (F30) solution (24% fructose, 28% dextrose) was added to the drinking water throughout pregnancy. At the end of pregnancy (day 19), blood, placenta, uterus, and fetuses were collected from rats. The results indicated that HFCS increases oxidative stress by increasing the level of MDA and decreasing GSH, SOD, and CAT activity in the blood of maternal. However, BA administration significantly decreased MDA levels and increased GSH levels, SOD, and CAT activity (p < 0.05). In addition, HFCS consumption significantly increased plasma TNF-alpha, IL-6, and leptin levels compared to control, BA, and HFCS + BA groups (p < 0.05). However, BA administration significantly decreased plasma TNF-alpha, IL-6, and leptin levels (p < 0.05). Furthermore, BA (20 mg/kg) significantly decreased HFCS-induced histopathological and immunohistochemical alterations in the placenta, uterus, and fetal tissue. In conclusion, BA may prevent HFCS toxicity in maternal and fetal tissues, as it regulates oxidative imbalance in pregnant rat and alleviates histopathological and immunohistochemical changes. The findings indicate a need for further studies to assess the potential of boron in preventing or mitigating the effects of HFCS during pregnancy.Article Citation - WoS: 1Citation - Scopus: 1The Protective Effect of Boric Acid Against High Fructose-Induced Liver and Kidney Damage in Rats(Springer, 2025-02-06) Yuksel, Duygu; Basegmez, Mehmet; Kan, FahriyeThis study aimed to determine the protective role of boric acid (BA) in high fructose (HF)-induced liver and kidney toxicity in a young rat model. High-fructose consumption causes serious damage to liver and kidney tissue in healthy individuals and contributes to the emergence of various metabolic diseases. Thirty-two healthy female Wistar albino rats (250–300 g weight and 3–4 months) were randomly distributed into four equal groups (n = 8): control, high fructose % 20 (HF), boric acid 20 mg/kg (BA), and HF + BA. High fructose was freshly prepared and administered to the rats as 20 g of d-fructose dissolved in 100 mL of tap water daily for a duration of 30 days. Boric acid (20 mg/kg) was administered through gastric gavage throughout the 30-day study period. At the end of study, blood, liver, and kidney were collected from rats. The results indicated that high fructose induced increased glucose, total cholesterol, triglyceride, and urea levels in rat serum. Boric acid administration significantly decreased glucose, total cholesterol, triglyceride, and urea levels in HF + BA groups. The results indicated that high fructose-induced oxidative stress by increasing the level of MDA and by decreasing GSH levels, and CAT activity in the liver and kidney of rats. However, oral BA administration significantly decreased MDA levels and increased GSH levels, and CAT activity (p < 0.05). Furthermore, BA significantly reduced high fructose-induced histopathological and Immunohistochemistry alteration in the liver and kidney tissues. In conclusion, BA may prevent the oxidative imbalance and histopathological and immunohistochemical damage caused by high fructose in liver and kidney tissues in rats. © The Author(s) 2025.Article Sıçanlarda Oral Yüksek Doz Vitamin C Takviyesinin Serum Vitamin C, Leptin ve Kortizol Parametrelerine Etkisi(2021-08-16) Eryavuz, Abdullah; Basegmez, MehmetBu çalışmanın amacı, sıçanlarda yüksek doz aralıklarında oral uygulanan vitamin C takviyesine bağlı olarak vitamin C, kortizol ve leptinin kan düzeylerindeki değişiklikleri belirlemektir. Toplam 21 adet 6-9 aylık yaşta Wistar Albino türü dişi sıçan rastgele olarak grup başına 7 hayvan içerecek s?ekilde üç gruba ayrıldı. Birinci grup kontrol grubu iken, deney grupları şu şekilde düzenlenmiştir: Vitamin C (1000mg/kg CA grubu, oral yoldan), Vitamin C (3000mg/kg CA grubu, oral yoldan). On günlük toplam deney süresi sonunda kan örnekleri İntrakardiak olarak serum tüplerine alındı. Serumda vitamin C, kortizol ve leptin düzeyleri belirlendi. Yapılan uygulamaların serum vitamin C, kortizol ve leptin düzeylerine etki etmediği bulundu. Sonuç olarak, oral olarak uygulanan yüksek doz vitamin C takviyesinin vücuttaki C vitamini havuzunu artırmanın etkili yolu olmadığı görüldü.