Acıpayam Meslek Yüksekokulu Koleksiyonu
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Article Effect of Carob Aqueous Extract on Boron, Copper, Zinc, Iron, and Vitamin C Levels in Brain, Heart, Liver, and Kidney Tissues of Diabetic Wistar Albino Rats(Wiley, 2026-03-29) Basegmez, Mehmet; Eryavuz, Azra Mila; Avsar, Mehmet Talha; Atik, Hulya Demirkapi; Demirel, Hasan Huseyin; Durmus, Ibrahim; Eryavuz, AbdullahThe utilization of medicinal plants in diabetes mellitus treatment is increasing owing to their effectiveness and safety compared to synthetic drugs. Thus, this study aimed to evaluate the effects of the aqueous extract of carob on tissue trace element levels, vitamin C concentrations, and histopathological alterations in streptozotocin-nicotinamide (STZ-NA)-induced diabetic Wistar albino rats. Rats were randomly divided into four experimental groups: control, diabetes, carob, and diabetes + carob. Following diabetes induction, rats in the diabetes + carob group were treated with carob aqueous extract during the experimental period. At the end of the study, tissue samples from the brain, liver, heart, and kidney were collected from rats. The samples were analyzed to determine vitamin C levels as well as trace elements, including boron (B), copper (Cu), zinc (Zn), and iron (Fe). Additionally, histopathological changes in collected tissues were examined. Diabetes significantly reduced vitamin C levels in brain and kidney tissues (p < 0.05), whereas no significant change was observed in the heart and liver (p > 0.05). Carob extract administration significantly increased tissue vitamin C levels in both diabetic and non-diabetic rats (p < 0.05). Additionally, both diabetes and carob aqueous extract administration were found to alter tissue trace mineral composition. In conclusion, the study demonstrates that carob aqueous extract can have an effect on tissue vitamin C and some trace mineral levels in normal and diabetic rats.Article Comparison of Selected Trace Elements, Oxidative Stress, and Antioxidant Status in Umbilical Cord Blood Plasma from Women in Rural and Urban Areas(Springer Nature, 2026-02-20) Basegmez, Mehmet; Yigit, Ayse Arzu; Basegmez, Elif; Simsek, OzkanThe aim of this study was to compare selected trace element levels in umbilical cord blood plasma of pregnant women residing in rural and urban areas and to investigate the relationship between trace elements and oxidative stress parameters. Umbilical cord blood samples were collected from 50 pregnant women living in rural and urban areas. Total antioxidant status (TAS) and total oxidant status (TOS) in plasma were determined by colorimetric method. Levels of nickel (Ni), manganese (Mn), aluminum (Al), iron (Fe), mercury (Hg), cadmium (Cd), vanadium (V), and selenium (Se) were measured by inductively coupled plasma optical emission spectrometry. Ni, Mn, Al, Fe, Hg, and Cd levels in cord blood plasma were significantly higher in pregnant women residing in rural areas compared to those in urban areas (p < 0.001). In contrast, V and Se concentrations were significantly higher in the urban group (p < 0.001). TAS and TOS levels in umbilical cord blood plasma were also significantly elevated in the rural group. The increased TAS levels observed in pregnant women residing in rural areas may reflect a compensatory and adaptive antioxidant response to elevated oxidative stress, as indicated by higher TOS levels, which may be associated with increased exposure to trace elements. This study provides preliminary data of significant differences in trace element levels, oxidative stress, and antioxidant status in umbilical cord blood plasma collected at delivery from women residing in rural and urban areas. These findings highlight environmental disparities between the two regions and provide a basis for future studies investigating prenatal exposure to trace elements and oxidative stress.Article Citation - WoS: 1Citation - Scopus: 1Effects of Vitamin C Supplementation on Total Antioxidant Status, Inflammation, and Histopathological Changes in Aged Rats(John Wiley and Sons Inc, 2025-05-30) Basegmez, Mehmet; Eryavuz, Abdullah; Demirel, Hasan HuseyinThis study aims to determine the effect of orally administered vitamin C (Vit C) supplementation on physiological and histopathological changes in aged rats of different genders. A total of 28 Sprague-Dawley aged male and female rats were randomly divided into four groups of seven animals per group. The study groups included the aged male control (MC), aged male with Vit C (MVC) (500 mg/kg vitamin C, orally) supplementation, female aged control (FC), and female aged with vitamin C (FVC) (500 mg/kg vitamin C, orally) supplementation groups. At the end of the study, which lasted 31 days, blood, brain, heart, liver, and kidney tissues were collected from rats under ketamine (87 mg/kg) and xylazine (13 mg/kg) anesthesia. The results indicated that although Vit C supplementation had no effect on serum Vit C levels, gender had an effect on serum Vit C levels (p < 0.05). However, Vit C supplementation and gender did not affect serum IL-6, IL-1β, TOS, and OSI levels (p > 0.05). Vit C supplementation, without the effect of gender, significantly increased TNF-α levels in MVC groups compared to MC groups (p < 0.05), while it significantly decreased them in FVC groups compared to FC groups (p < 0.05). In addition, Vit C significantly reduced histopathological alterations in brain, heart, and liver tissues associated with aging, including oxidative stress and inflammation. In conclusion, it was observed that orally administered 500 mg/kg Vit C supplementation to old rats is not an effective way to increase the Vit C pool in the body, but gender has an impact on the blood Vit C concentrations. © 2025 The Author(s). Journal of Biochemical and Molecular Toxicology published by Wiley Periodicals LLC.Article Citation - WoS: 6Citation - Scopus: 5Propolis Extract Reduces Doxorubucin-Induced Brain Damage by Regulating Inflammation, Er Stress, Oxidative Stress, and Apoptosis(John Wiley and Sons Inc, 2025-04) Gelen, Volkan; Basegmez, Mehmet; Dursun, Inan; Cinar, Irfan; Kara, AdemDoxorubicin (DOX) is the most widely used chemotherapeutic agent to treat various tumors. DOX treatment can damage many organs, including the brain, by causing oxidative stress. Several antioxidant substances can lessen the effects of DOX or make antioxidant defense systems work faster. Propolis (PROP) is a powerful agent with various healing effects, including antioxidant, antiproliferative, and anti-inflammatory. The point of this study is to look at the histopathological changes, apoptosis, and antioxidant effects of DOX on brain damage in rats. To find out what kinds of phytochemicals were in PROP from the Karlıova region of Bingöl province, ultra-high-performance liquid chromatography (UHPLC-Orbitrap-HRMS) was used. Then, we made an ethanol extract of it. A total of 28 healthy male Wistar albino rats, each 12 weeks old and weighing between 220 and 250 g, were included in the study. Rats were divided into four groups: control, PROP, DOX, and PROP+DOX. We applied the relevant treatments to the determined groups. Following the application, we decapitated the rats under the appropriate conditions and collected blood and brain tissue samples. We examined oxidative stress parameters in blood samples and used brain tissue samples for histopathological, biochemical, and molecular analyses. We determined DOX levels in the brain tissue samples using UHPLC-Orbitrap-HRMS. The findings obtained showed that the PROP extract improved DOX-induced brain tissue damage. In addition, PROP extract attenuated DOX-induced brain tissue inflammation, ER stress, apoptosis, and oxidative stress. © 2025 The Author(s). Food Science & Nutrition published by Wiley Periodicals LLC.Article Citation - WoS: 2Citation - Scopus: 3The effect of boric acid on oxidative stress, inflammation, and apoptosis in embryonic and fetal tissues damage caused by consumption of high-fructose corn syrup in pregnant rats(Springer Heidelberg, 2025-01-16) Basegmez, Mehmet; Yuksel, DuyguThis study aimed to determine the protective role of boric acid in a pregnant rat model of high fructose corn syrup consumption. Consumption of high fructose corn syrup has been associated with adverse health outcomes in humans and animals. Twenty-eight healthy female Wistar albino rats (250-300 g weight and 16-24 weeks old) were randomly distributed into four equal groups (n = 7): Control, Boric acid (BA), High Fructose Corn Syrup (HFCS), HFCS + BA. Boric acid (20 mg/kg) was administered to pregnant rats via oral gavage every day during pregnancy. The prepared 30% HFCS (F30) solution (24% fructose, 28% dextrose) was added to the drinking water throughout pregnancy. At the end of pregnancy (day 19), blood, placenta, uterus, and fetuses were collected from rats. The results indicated that HFCS increases oxidative stress by increasing the level of MDA and decreasing GSH, SOD, and CAT activity in the blood of maternal. However, BA administration significantly decreased MDA levels and increased GSH levels, SOD, and CAT activity (p < 0.05). In addition, HFCS consumption significantly increased plasma TNF-alpha, IL-6, and leptin levels compared to control, BA, and HFCS + BA groups (p < 0.05). However, BA administration significantly decreased plasma TNF-alpha, IL-6, and leptin levels (p < 0.05). Furthermore, BA (20 mg/kg) significantly decreased HFCS-induced histopathological and immunohistochemical alterations in the placenta, uterus, and fetal tissue. In conclusion, BA may prevent HFCS toxicity in maternal and fetal tissues, as it regulates oxidative imbalance in pregnant rat and alleviates histopathological and immunohistochemical changes. The findings indicate a need for further studies to assess the potential of boron in preventing or mitigating the effects of HFCS during pregnancy.Article Citation - WoS: 1Citation - Scopus: 1The Protective Effect of Boric Acid Against High Fructose-Induced Liver and Kidney Damage in Rats(Springer, 2025-02-06) Yuksel, Duygu; Basegmez, Mehmet; Kan, FahriyeThis study aimed to determine the protective role of boric acid (BA) in high fructose (HF)-induced liver and kidney toxicity in a young rat model. High-fructose consumption causes serious damage to liver and kidney tissue in healthy individuals and contributes to the emergence of various metabolic diseases. Thirty-two healthy female Wistar albino rats (250–300 g weight and 3–4 months) were randomly distributed into four equal groups (n = 8): control, high fructose % 20 (HF), boric acid 20 mg/kg (BA), and HF + BA. High fructose was freshly prepared and administered to the rats as 20 g of d-fructose dissolved in 100 mL of tap water daily for a duration of 30 days. Boric acid (20 mg/kg) was administered through gastric gavage throughout the 30-day study period. At the end of study, blood, liver, and kidney were collected from rats. The results indicated that high fructose induced increased glucose, total cholesterol, triglyceride, and urea levels in rat serum. Boric acid administration significantly decreased glucose, total cholesterol, triglyceride, and urea levels in HF + BA groups. The results indicated that high fructose-induced oxidative stress by increasing the level of MDA and by decreasing GSH levels, and CAT activity in the liver and kidney of rats. However, oral BA administration significantly decreased MDA levels and increased GSH levels, and CAT activity (p < 0.05). Furthermore, BA significantly reduced high fructose-induced histopathological and Immunohistochemistry alteration in the liver and kidney tissues. In conclusion, BA may prevent the oxidative imbalance and histopathological and immunohistochemical damage caused by high fructose in liver and kidney tissues in rats. © The Author(s) 2025.Article Citation - WoS: 12Citation - Scopus: 11The effect of vitamin C supplementation on favipiravir-induced oxidative stress and proinflammatory damage in livers and kidneys of rats(Taylor & Francis Ltd, 2023-02-22) Dogan, Muhammed Fatih; Kaya, Kursat; Demirel, Hasan Huseyin; Basegmez, Mehmet; Sahin, Yasemin; Ciftci, OsmanBackground: Favipiravir (FPV), an effective antiviral agent, is a drug used to treat influenza and COVID-19 by inhibiting the RNA-dependent RNA polymerase (RdRp) of RNA viruses. FPV has the potential to increase oxidative stress and organ damage. The purpose of this study was to demonstrate the oxidative stress and inflammation caused by FPV in the liver and kidneys of rats, as well as to investigate the curative effects of vitamin C (VitC).Methods: A total of 40 Sprague-Dawley male rats were randomly and equally divided into the following five groups: 1st; Control, 2nd; FPV = 20 mg/kg, 3rd; FPV = 100 mg/kg, 4th; FPV = 20 mg/kg + VitC (150 mg/kg), and 5th; FPV = 100 mg/kg + VitC (150 mg/kg) groups. Rats were given either FPV (orally) or FPV plus VitC (intramuscular) for 14 days. Rat blood, liver, and kidney samples were collected at 15 days to be analyzed for oxidative and histological changes.Results: FPV administration resulted in an increase in proinflammatory cytokines (TNF-alpha and IL-6) in the liver and kidney, as well as oxidative and histopathologic damage. FPV increased TBARS levels significantly (p < .05) and decreased GSH and CAT levels in liver and kidney tissues but had no effect on SOD activity. VitC supplementation significantly reduced TNF-a, IL-6, and TBARS levels while increasing GSH and CAT levels (p < .05). Furthermore, VitC significantly attenuated FPV-induced histopathological alterations associated with oxidative stress and inflammation in the liver and kidney tissues (p < .05).Conclusion: FPV caused liver and kidney damage in rats. In contrast, co-administration of FPV with VitC improved FPV-induced oxidative, pro-inflammatory, and histopathological changes.Article Sıçanlarda Oral Yüksek Doz Vitamin C Takviyesinin Serum Vitamin C, Leptin ve Kortizol Parametrelerine Etkisi(2021-08-16) Eryavuz, Abdullah; Basegmez, MehmetBu çalışmanın amacı, sıçanlarda yüksek doz aralıklarında oral uygulanan vitamin C takviyesine bağlı olarak vitamin C, kortizol ve leptinin kan düzeylerindeki değişiklikleri belirlemektir. Toplam 21 adet 6-9 aylık yaşta Wistar Albino türü dişi sıçan rastgele olarak grup başına 7 hayvan içerecek s?ekilde üç gruba ayrıldı. Birinci grup kontrol grubu iken, deney grupları şu şekilde düzenlenmiştir: Vitamin C (1000mg/kg CA grubu, oral yoldan), Vitamin C (3000mg/kg CA grubu, oral yoldan). On günlük toplam deney süresi sonunda kan örnekleri İntrakardiak olarak serum tüplerine alındı. Serumda vitamin C, kortizol ve leptin düzeyleri belirlendi. Yapılan uygulamaların serum vitamin C, kortizol ve leptin düzeylerine etki etmediği bulundu. Sonuç olarak, oral olarak uygulanan yüksek doz vitamin C takviyesinin vücuttaki C vitamini havuzunu artırmanın etkili yolu olmadığı görüldü.Article Effects of Age on The Concentrations of Plasma Cytokinesand Lipidperoxidation in Sheep(2021-02-09) Durmus, Ibrahim; Küçükkurt, Ismail; Eryavuz, Abdullah; Basegmez, Mehmet; Salim, Mehmet NaciThe aim of this study is to determine the effectsof age on blood cytokine levels and lipid peroxidationproductmalondialdehyde (MDA) levels and thyroid hormones levels in sheep of different ages. Female sheep oflamb (4-6months), young (12-18 months) and adult (36-40 months) from a herd in same care and feeding conditions wereused in the study. Blood samples were taken before morning feding from 30 sheep in total, 10 heads from eachage group. MDA, glutathione (GSH), interleukin-1 (IL-1), tumor necrosis factor alpha (TNF-?), interleukin-6(IL-6) and T3 and T4 levels were determined in blood samples. Young animals have significantly higher MDAand lower IL-6 levels than lambs. There was no difference between age groups in terms of GSH, IL-1?, TNF-?,T3 and T4 levels. As a result, age has an effect on blood lipid peroxidation and IL-6 levels in sheep.Article Determination of The Effects of 2,4-Dichlorophenoxy Acetic Acid on Hematological Parameters in Rats Given Alcohol(2020) Durmus, Ibrahim; Eryavuz, Abdullah; Basegmez, Mehmet; Etyemez, MuhammedIn modern agriculture, many compounds such as pesticides, herbicides and various kinds of fertilizers are frequently used inthe fields to increase crop production. However, among these compounds, widespread usage of synthetic herbicides foragricultural purpose has an adverse effect on living organisms and can lead to several hematological and neurologicalcomplications in human and animals. Alcohol consumption is one of the potential risk factors in cardiovascular disease. Theaim of this study is to investigate the effects of 2,4-dichlorophenoxy acetic acid (2.4-D), used commonly by farmers as aherbicide, on hematological changes in rats given alcohol. Totally 28 Sprague Dawley male rats were randomly divided intofour groups containing 7 animals per group. While first group served as a control, experimental groups were as follows;herbicide group (5 mg/kg, 2,4- D, orally), alcohol group (3 mg/kg ethyl alcohol, orally), herbicide plus alcohol group (5mg/kg 2,4-D and 3 mg/kg ethyl alcohol, orally). In the end of experimental period lasted totally 60 days, the blood sampleswere taken from animals by cardiac puncture under anesthesia. In the blood samples, erythrocyte (RBC), total leukocytes(WBC) and percentage of the different white blood cells, hematocrit value (HCT), hemoglobin concentration (HGB) andplatelet counts (PLT) were determined. 2,4-D increased the WBC, percentage of lymphocyte, monocyte and granulocytenumbers. Alcohol and alcohol plus 2,4-D application reduced RBC numbers, HGB, HCT value and PLT concentration.The mean corpuscular hemoglobin level (MCH) was increased by alcohol, 2.4 D and alcohol plus 2.4 D treatments. Weconcluded that 2,4-D application in rats given alcohol leads to changes in hematological parameters.