Diş Hekimliği Fakültesi Koleksiyonu
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Article Citation - WoS: 2Citation - Scopus: 2The Effects of Abaloparatide on Alveolar Bone Loss in Postmenopausal Osteoporotic Rats with Periodontitis(BMC, 2025-09-29) Kizildag, Alper; Alpan, Aysan Lektemur; Ozdogan, Zeynep; Ozmen, Ozlem; Zavrak, Necati; Terzi, MithatBackground: Abaloparatide is a second-generation osteoporosis drug that prevents bone loss and stimulates bone fromation. This study evaluated the effects of abaloparatide treatment on alveolar bone loss (ABL) in osteoporotic rats with periodontitis by immunohistochemical and microcomputed tomography (micro-CT) in rats with periodontitis and osteoporosis. Methods: Forty adult female rats were divided into five equal groups: non-ligated (controls), periodontitis (P), periodontitis + osteoporosis (PO), PO treatment with 10 mu g/kg abaloparatide (PO-10), and PO treatment with 80 mu g/kg abaloparatide (PO-80). Bilateral ovariectomy was conducted in the osteoporosis groups. Three weeks after ovariectomy, experimental periodontitis was induced via Ligature. Abaloparatide was performed at the same time as the induction of experimental periodontitis. All rats were sacrificed at 30 days. ABL was determined via micro-CT and histological analyses. Bone morphogenetic protein 2 (BMP-2), alkaline phosphatase (ALP), receptor ligand for nuclear factor-kappa B (RANKL), osteocalcin (OCN), osteoprotegerin (OPG), and collagen 1 (Col-1) were evaluated immunohistochemically. In addition, the levels of C-terminal telopeptide of type I collagen (CTX), which is related to bone turnover, were analyzed. Results: Periodontitis with osteoporosis significantly increased ABL in the PO versus the P group. Abaloparatide treatment significantly reduced ABL in the treatment groups and significantly increased BMP-2, ALP, Col-1, OPG, OCN, and CTX levels. In particular, 80 mu g/kg abaloparatide treatment significantly modulated the RANKL, OPG, and CTX levels in the PO-80 group. The micro-CT results indicated that abaloparatide treatment significantly enhanced the percentage of bone, trabecular thickness, trabecular number, and bone surface density. Conclusion: Abaloparatide treatment regulates bone formation dose-dependently by decreasing ABL in rats with periodontitis and osteoporosis.Article Citation - WoS: 1Citation - Scopus: 1The Effects of Local and Systemic Metronidazole on Bone Healing in Mandibular Defects: a Rat Model(BioMed Central Ltd, 2025-07-02) Alpan, Aysan Lektemur; Kizildag, Alper; Ozmen, Ozlem; Zavrak, Necati; Ozdogan, Zeynep; Akpinar, AysunObjective: This study evaluated the effects of local and systemic Metronidazole (MTZ) on bone healing in infected mandibular defects using a rat model. Study design: Thirty Wistar rats were divided into Control, Local MTZ (L-MTZ), and Systemic MTZ (S-MTZ) groups. Mandibular defects were treated with xenograft mixed saliva from periodontitis patients. Bone regeneration was evaluated using micro-CT and histological analyses. Bone morphogenic protein-2 (BMP-2), alkaline phosphatase (ALP), receptor activator of nuclear factor kappaB ligand (RANKL), osteocalcin (OCN), and beta-catenin (β-catenin), high sensitivity C-reactive protein (hs-CRP) levels evaluated. Results: Increased bone formation and reduced osteoclast counts were detected in the L-MTZ group compared to the Control. β-catenin expression was significantly higher in the L-MTZ group, BMP-2 and Runx2 levels were elevated in both L-MTZ and S-MTZ groups. ALP and OCN levels were the highest in the L-MTZ group, with no significant difference between the L-MTZ and S-MTZ groups. hs-CRP levels were significantly lower in MTZ-treated groups. Micro-CT analysis revealed the highest bone volume/total volume (BV/TV) ratio in the S-MTZ group among all groups. Conclusion: Local MTZ application enhanced bone regeneration by promoting osteoblast activity, activating β-catenin and BMP-2/Runx2 signaling, and reducing inflammation. Systemic MTZ also improved bone healing, particularly in volumetric aspects. © 2025 Elsevier B.V., All rights reserved.Article Citation - WoS: 1Citation - Scopus: 1Assessment and comparative study of diosgenin doses in alleviating experimental periodontitis(BMC, 2024-07-28) Kizildag, Alper; Alpan, Aysan Lektemur; Aydin, Tugba Koseoglu; Ozdede, Melih; Ozmen, OzlemBackground This study was performed to determine the therapeutic effects of diosgenin (DG) which is a steroidal saponin, administered at different doses on alveolar bone loss (ABL) in rats with experimental periodontitis using immunohistochemical and cone-beam computed tomography (CBCT). Methods Thirty-two male Wistar rats divided into four equal groups: control (non-ligated), periodontitis (P), DG-48, and DG-96. Sutures were placed at the gingival margin of the lower first molars to induce experimental periodontitis. Then, 48 and 96 mg/kg of DG was administered to the study groups by oral gavage for 29 days. At day 30, the animals were sacrificed and ABL was determined via CBCT. The expression patterns of osteocalcin (OCN), alkaline phosphatase (ALP), type I collagen (Col-1), B cell lymphoma 2 (Bcl 2), Bcl 2-associated X protein (Bax), bone morphogenetic protein 2 (BMP-2), and receptor activator of NF kappa B ligand (RANKL) were examined immunohistochemically. Results Histopathologic examination showed all features of the advanced lesion in the P group. DG use decreased all these pathologic changes. It was observed that periodontitis pathology decreased as the dose increased. DG treatment increased the ALP, OCN, Bcl 2, Col-1, and BMP-2 levels in a dose-dependent manner, compared with the P group (p < 0.05). DG decreased the expression of RANKL and Bax in a dose-dependent manner (p < 0.05). ABL was significantly lower in the DG-48 and DG-96 groups than in the P group (p < 0.05). Conclusion Collectively, our findings suggest that DG administration protects rats from periodontal tissue damage with a dose-dependent manner, provides an increase in markers of bone formation, decreases in Bax/Bcl-2 ratio and osteoclast activation.Article Citation - WoS: 8Citation - Scopus: 9Efficacy of injectable platelet-rich fibrin on clinical and biochemical parameters in non-surgical periodontal treatment: a split-mouth randomized controlled trial(Springer Science and Business Media Deutschland GmbH, 2023-12-28) Cin, Gizem Torumtay; Alpan, Aysan Lektemur; Cevik, OezgeAbstract: Objectives: The purpose of this clinical trial was to evaluate the potential clinical and biochemical effects of injectable platelet-rich fibrin (i-PRF) application adjunct to scaling and root planning (ScRp) in deep periodontal pockets. Materials and methods: In this split-mouth-designed study, 17 patients with 34 deep periodontal pockets were randomly treated with ScRp + i-PRF (test group) and ScRp + saline (control group). Clinical periodontal measurements were recorded at baseline, 1st, 3rd, and 6th months after the treatments. The levels of vascular endothelial growth factor (VEGF), tumor necrosis factor-α (TNF-α), and interleukin (IL)-10 in gingival crevicular fluid (GCF) samples were analyzed using the ELISA method at baseline, 7th, and 14th days. Results: Clinical periodontal parameters showed significant improvements with both treatment modalities. Mean pocket reduction (PD) and clinical attachment (CAL) gain were significantly higher in the test group than in controls at follow-up visits (p < 0.05). In the test group, gingival recession (GR) values were significantly lower compared to the control group. VEGF and IL-10 levels in the test group were significantly higher than in controls at the 14th day, and TNF-α levels were found significantly lower in the test group at the 7th and 14th days. Conclusions: Especially in the test group, the significant increase in VEGF and IL-10 expressions and the decrease in TNF-α levels may have accelerated the periodontal healing observed in the clinical parameters. Clinical relevance: The result of the present study demonstrated the beneficial effects of adjunctive i-PRF administration during non-surgical periodontal treatment of deep periodontal pockets. Clinical trial registration number: NCT05753631. © 2023, The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.Article Citation - WoS: 1Citation - Scopus: 3Articaine versus lidocaine inferior alveolar nerve block in posterior mandible implant surgeries: a randomized controlled trial(Medicina Oral S L, 2023) Gulnahar, Yakup; Alpan, Aysan Lektemur; Gulnahar, EvremBackground: The aim of this study is to compare the effects of %4 articaine and %2 lidocaine on inferior alveolar nerve block (IANB) for implant surgery in the posterior mandible.Material and Methods: The patients who have inserted implants in the posterior mandible were divided into 2 groups for IANB: lidocaine and articaine. VAS = visual analog scale, pain during surgery and injection, lip numb-ness time, mandibular canal-implant apex distance, age, gender, bone density, implant number, release incision, adjacent teeth, and duration of surgery were analyzed using t-test, Mann-Whitney U test, Spearman's coefficient, and, Pearson's chi-squared test. This trial followed the recommendations of the Consort Statement for reporting randomized controlled trials. Results: 577 patients were included and 1185 dental implants were analyzed. There was no significant difference between the two groups in terms of injection and surgery VAS values (p>0.05). The lip numbness time of lido-caine was 3.06 +/- 3.22min while articaine was found to be 2.96 +/- 3.09min (p>0.05). Mandibular canal-implant apex distance was found to be 2.28 +/- 0.75mm in the articaine and 2.45 +/- 0.86mm in the lidocaine group (p<0.05). Release incision was made more in the articaine group (51/252) than in the lidocaine group (40/325) (p<0.05).Conclusions: There was no difference between the %4 articaine and %2 lidocaine in terms of pain perception in posterior mandible implant applications. Both anesthetics provided adequate anesthesia for implant application.Article Periodontal Parameters And Periodontitis Severity In Type 2 Diabetes Patients With Chronic Complications(Annals Dental Specialty, 2022) Cin, Gizem Torumtay; Fenkci, Semin Melahat; Yigit, Umut; Alpan, Aysan LektemurLong-term hyperglycemia is related to micro and/or macrovascular complications in diabetes mellitus. The interrelationship between diabetes and periodontitis is bi-directional and inflammation is the common feature of both diseases. This study aimed to search the effects of the number of chronic vascular complications in type 2 diabetics on the amount of periodontal tissue destruction and the periodontal disease severity. This cross-sectional study included 127 individuals with type 2 diabetes who developed at least one diabetes-related chronic complication. The severity of periodontitis and the amount of gingival inflammation and periodontal tissue destruction between patients with several vascular complications were compared. We also compared the periodontal parameters and the severity of periodontitis according to glycemic control levels and in terms of the preŞence of macrovascular complications. All periodontal clinical parameters and periodontal disease severity were significantly higher in patients with 3 and 4 diabetic vascular complications than in patients with fewer complications. In patients without macrovascular complications, the severity of periodontitis was significantly lower compared to those with macrovascular complications (p<0.05). WorŞened gingival inflammation was shown in patients with poor glycemic control, but there was no statistically significant difference in PD and CAL. Our findings showed that the severity of periodontal disease was higher as the number of diabetes-related chronic complications increased. The extent of gingival inflammation was elevated with poor glycemic control whereas the amount of periodontal tissue destruction did not change.Article Citation - WoS: 21Citation - Scopus: 21Comparison of hyaluronic acid, hypochlorous acid, and flurbiprofen on postoperative morbidity in palatal donor area: a randomized controlled clinical trial(Springer Heidelberg, 2023-01-03) Alpan, Aysan Lektemur; Cin, Gizem TorumtayObjectiveThis study aims to evaluate the effects of topical hyaluronic acid (HA), hypochlorous acid (HOCl), and flurbiprofen on postoperative morbidity of palatal donor sites after free gingival graft (FGG) surgery. Materials and methodsSixty patients requiring FGG were randomly assigned into four groups: control, HA gel (600 mg/100 g high molecular weight hyaluronic acid), HOCl spray (170-200 ppm, ph7.1), flurbiprofen spray (0.075gr flurbiprofen). Topical agents were applied for 14 days, according to groups. Patients were followed for 28 days. Palatal healing was assessed with the Laundry wound healing index (WHI). Complete epithelization (CE) was evaluated with photographs and H2O2 bubbling. Pain, burning Şensation, chewing efficacy, and tissue color match (CM) were evaluated using a visual analog scale (VAS). Postoperative analgesic consumption and delayed bleeding (DB) were also recorded. ResultsHA provided better WHI values on the 7(th), 14(th), and 21(st) days compared to the other groups, respectively (p < 0.05). CE was formed on the 21(st) day in the HA group but on the 28(th) day in the other groups. HOCl and flurbiprofen groups were not different from the control group or each other in terms of WHI. HOCl had the lowest VAS scores of all time periods. DB was not observed in any group. Significantly fewer analgesics were taken in the topical agent-applied groups compared to the control group. ConclusionsHA exhibits a positive impact on the epithelization of palatal wound healing and color matching. HOCl and flurbiprofen provided less pain; however, they might have negative effects on palatal wound healing.Article Citation - WoS: 56Citation - Scopus: 60PRF improves wound healing and postoperative discomfort after harvesting subepithelial connective tissue graft from palate: a randomized controlled trial(Springer, 2019-05-18) Alpan, Aysan Lektemur; Cin, Gizem TorumtayObjectives: The aim of this study is to determine the use of platelet-rich fibrin (PRF) in the management of soft tissue donor site healing after harvesting connective tissue graft (CTG) from the palate and evaluate the postoperative discomfort (pain, bleeding, analgesic consumption, tissue color match) of patients. Materials and methods: Forty patients were randomly assigned to PRF or control group. In the PRF group, PRF membrane was placed into CTG donor site. After surgery, delayed bleeding, early healing index (EHI), tissue color match, and analgesic consumption were recorded. The visual analog scale (VAS) was used to observe the postoperative pain and tissue color match. Data were analyzed using the independent sample t test and the repeated measure ANOVA test. Results: The patients in the PRF group reported significantly lower pain scores at all-time points. Postoperative 3rd and 7th day, EHI scores were lower in the favor of the PRF group. VAS score values of tissue color match were lower in the control group at 7th and 14th day, compared with the PRF group. Analgesic intake was significantly lower in the PRF group postoperatively at 1st and 3rd day that of control group. Conclusion: PRF application at the palatal donor site demonstrates promising results in terms of better wound healing and reduced postoperative discomfort in the patients after harvesting CTG. Clinical relevance: Patients may avoid surgical operations because of the discomfort or pain feeling. Reducing postoperative pain and discomfort and accelerating recovery meet the wishes of every patient and physician. PRF can provide these requirements as an easy method to obtain and implement. © 2019, Springer-Verlag GmbH Germany, part of Springer Nature.Article Citation - WoS: 19Citation - Scopus: 20The effects of taxifolin on alveolar bone in experimental periodontitis in rats(Elsevier Ltd, 2020-09) Alpan, Aysan Lektemur; Kizildag, Alper; Ozdede, Melih; Karakan, Nebi Cansin; Ozmen, OzlemObjective: This study aimed to evaluate the effect of taxifolin, a powerful antioxidant, on the progression of periodontitis by immunohistochemical and cone-beam computed tomography (CBCT) examination. Design: This study was performed with 32 rats in four experimental groups: a non-ligated group (Control, n = 8), periodontitis group (Perio, n = 8), periodontitis with 1 mg/kg/day taxifolin group (Taxi-1, n = 8), and periodontitis with 10 mg/kg/day taxifolin group (Taxi-10, n = 8). A ligature-induced experimental periodontitis design was used. All rats were sacrificed at 30 days. Alveolar bone loss was determined by CBCT. Hematoxylin–eosin stained slides were examined. The expression levels of bone morphogenetic protein 2 (BMP-2), osteocalcin (OCN), alkaline phosphatase (ALP), collagen type I (Col 1), Bcl-2, Bax, and receptor activator of NF-?B ligand (RANKL) were determined immunohistochemically. Results: Both doses of taxifolin showed a decrease in alveolar bone loss. The inflammatory reaction was higher in the Perio group and lower in the taxifolin groups. BMP-2, OCN, ALP, and Col 1 expression were dose-dependently elevated in the taxifolin groups. RANKL immunoexpression decreased with both doses of taxifolin. Bcl-2 expression increased and Bax expression decreased in the taxifolin groups. Conclusion: Taxifolin successfully reduced apoptosis and improved bone formation in alveolar bone in this experimental periodontitis model. © 2020 Elsevier LtdArticle Citation - WoS: 15Citation - Scopus: 21Humic Acid Enhances Wound Healing in the Rat Palate(Hindawi Limited, 2018-01) Calisir, Metin; Akpinar, Aysun; Talmac, Ahmet Cemil; Alpan, Aysan Lektemur; Goze, Omer FahrettinIntroduction. Humic acid was previously shown to enhance cutaneous wound healing and show antibacterial properties; however, it has not been used for wound healing in the oral cavity. Thus, the goal of this study was the evaluation of the effect of the humic acid on the healing of excisional wounds in an experimental rat study. Materials and Methods. A circular wound on mid-palatal surfaces was made on a total of 77 Wistar rats by using a 3-mm biopsy punch under anesthesia. The animals were divided into 4 groups as baseline, saline control (0.09%), chlorhexidine gluconate (0.05%), and humic acid (80 mg/kg) and were treated with these materials for 7 days. Results. The rats were observed for 3 weeks in order to track the wound closure rates. Both humic acid treatment and chlorhexidine gluconate treatment resulted in statistically significant enhanced rate of wound closure compared to the saline control on both the 1st and 2nd weeks of treatment. Humic acid treatment for the wounds in the palate resulted in enhanced recovery compared to not only saline control but also chlorhexidine gluconate treatment. Conclusion. In this study, humic acid was shown to enhance healing of oral wounds for the first time in the literature. These findings indicate that humic acid can be used as an alternative to current treatment methods for oral wounds. © 2018 Metin Çalişir et al.