Please use this identifier to cite or link to this item:
https://hdl.handle.net/11499/10383
Title: | The effect of nesfatin-1 on heart L-type Ca2+ channel a?1c subunit in rats subjected to chronic restraint stress | Authors: | Ayada, C. Turgut, Günfer Turgut, S. |
Keywords: | Cardiac failure Chronic restraint stress L-type Ca2+ channel ?1c subunit Nesfatin-1 Western blot Cacna1c protein, rat calcium binding protein calcium channel L type DNA binding protein nerve protein nucleobindin animal drug effects exercise heart heart muscle male mental stress metabolism rat Wistar rat Animals Calcium Channels, L-Type Calcium-Binding Proteins DNA-Binding Proteins Heart Male Myocardium Nerve Tissue Proteins Rats Rats, Wistar Restraint, Physical Stress, Psychological |
Publisher: | Comenius University | Abstract: | Background: Elevated L-type Ca2+ channel expression level increases Ca2+ influx. This can cause hypertrophy and pathological remodeling of the heart especially under stress conditions. Nesfatin-1 can activate hypothalamic L, P and Q type Ca2+ channels and increase insulin secretion in pancreatic islet beta cells via activation of L-type Ca2+ channels. On the other hand, the effect of nesfatin-1 on cardiac L-type Ca2+ channels has not been studied yet. Objectives: We aimed to identify the effect of peripheral chronic nesfatin-1 application on cardiac L-type Ca2+ channel ?1c subunit expression level in normal rats and those subjected to chronic restraint stress. Methods: Three-month aged Wistar albino rats were randomly divided into 4 groups (n = 7) as Control, Stress, Control+Nesfatin-1, and Nesfatin-1+Stress. Rats in groups subjected to restraint stress were placed in a specially built size-manipulable cabin for 2 h/day (between 10:00 and 12:00 a.m.) for 10 consecutive days without allowing water and food intake. Nesfatin-1 segment (0.25 nmol/g bw intraperitoneally) was applied during the 10 consecutive days. Western blot analyses were performed to determine the expression level of L-type Ca2+ channel ?1c subunit protein in rat cardiac extracts. Results: Cardiac L-type Ca2+ channel ?1c subunit protein expression levels were increased significantly after chronic peripheral nesfatin-1 application in rats subjected to restraint stress (p = 0.032). Conclusion: We can conclude that nesfatin-1 can cause cardiac failures during clinical treatments by elevating cardiac L-type Ca2+ channel ?1c subunit protein expression level (Fig. 2, Ref. 26). Text in PDF www.elis.sk. | URI: | https://hdl.handle.net/11499/10383 https://doi.org/10.4149/BLL_2015_061 |
ISSN: | 0006-9248 |
Appears in Collections: | PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection Tıp Fakültesi Koleksiyonu WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection |
Files in This Item:
File | Size | Format | |
---|---|---|---|
Effect.pdf | 303.38 kB | Adobe PDF | View/Open |
CORE Recommender
SCOPUSTM
Citations
3
checked on Jun 29, 2024
WEB OF SCIENCETM
Citations
2
checked on Jul 17, 2024
Page view(s)
30
checked on May 27, 2024
Download(s)
8
checked on May 27, 2024
Google ScholarTM
Check
Altmetric
Items in GCRIS Repository are protected by copyright, with all rights reserved, unless otherwise indicated.