Please use this identifier to cite or link to this item: https://hdl.handle.net/11499/11019
Title: Prevalence and antimicrobial resistance of thermophilic Campylobacter isolates from raw chicken meats
Authors: Issa, G.
Basaran Kahraman, B.
Adiguzel, M.C.
Yilmaz Eker, F.
Akkaya, E.
Bayrakal, G.M.
Koluman, Ahmet
Kahraman, Tolga
Keywords: Antimicrobial resistance
Campylobacter
PCR
Prevalence
Raw chicken meat
ciprofloxacin
erythromycin
gentamicin
kanamycin
nalidixic acid
tetracycline
animal tissue
antibiotic resistance
antibiotic sensitivity
antimicrobial resistance gene
Article
bacterium identification
bacterium isolation
broth dilution
Campylobacter coli
Campylobacter jejuni
chicken meat
food contamination
gene
genetic analysis
minimum inhibitory concentration
mismatch amplification mutation assay
multiplex polymerase chain reaction
nonhuman
prevalence
Publisher: Veteriner Fakultesi Dergisi
Abstract: Globally, the spread of antibiotic resistance via chicken meat consumption cause serious public health concerns. With this respect, the current study aimed to investigate the prevalence of thermophilic Campylobacter species isolated from raw meat chicken samples and their genetic determinants of resistance to various classes of antibiotics. A total of 540 chicken raw meat samples collected from various supermarkets and slaughterhouses in Istanbul, Turkey were analyzed according to EN ISO 10272-1:2006 standard procedure. For identification of the genus and species of the isolates, multiplex PCR assay was held. Minimum inhibitory concentrations of the antimicrobial agents (nalidixic acid, ciprofloxacin, tetracycline, gentamicin, kanamycin, and erythromycin) were initially determined using the broth microdilution method. In addition, the genetic determinants of antimicrobial resistance were investigated by PCR assays. In total, 357 (66.1%) Campylobacter isolates were obtained including 268 Campylobacter jejuni and 89 Campylobacter coli. Resistance to quinolones (nalidixic acid and ciprofloxacin) was the most common in all strains (80.1%), followed by resistance to tetracycline’s (70.3%). The lowest resistance was determined as resistance to kanamycin (4.2%). Gentamicin and erythromycin resistance was not observed in this study. Only five C. coli isolate (1.4%) was classified as multidrug resistant. On the basis of these data, execute widely presence of antimicrobial resistance to quinolones and tetracycline’s in C. jejuni and C. coli isolates from chicken raw meat samples and emphasizes that further multidisciplinary studies and novel strategies in the concept of ‘One Health’ are needed. © 2018, Veteriner Fakultesi Dergisi. All rights reserved.
URI: https://hdl.handle.net/11499/11019
https://doi.org/10.9775/kvfd.2018.19741
ISSN: 1300-6045
Appears in Collections:Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
Teknoloji Fakültesi Koleksiyonu
TR Dizin İndeksli Yayınlar Koleksiyonu / TR Dizin Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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