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https://hdl.handle.net/11499/26755
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DC Field | Value | Language |
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dc.contributor.author | Şen, Alaattin | - |
dc.contributor.author | Ayar, Buket | - |
dc.contributor.author | Yılmaz, Anıl | - |
dc.contributor.author | Özgün Acar, Özden | - |
dc.contributor.author | Çelik Turgut, Gurbet | - |
dc.contributor.author | Topçu, Gülaçtı | - |
dc.date.accessioned | 2019-10-09T10:58:59Z | - |
dc.date.available | 2019-10-09T10:58:59Z | - |
dc.date.issued | 2019-10-07 | - |
dc.identifier.issn | 1300-0527 | - |
dc.identifier.uri | https://hdl.handle.net/11499/26755 | - |
dc.identifier.uri | https://doi.org/10.3906/kim-1904-57 | - |
dc.description.abstract | Teucrium species have been used in folk medicine as antidiabetic, antiinflammatory, antiulcer, and antibacterial agents. We have explored in vitro antidiabetic impacts of 2 natural diterpenoids, alysine A and alysine B, isolated from Teucrium alyssifolium. The lactate dehydrogenase (LDH) cytotoxicity assay, glucose uptake test, glucose utilization (glycogen content) test, glucose transport test, glucose absorption (?-glucosidase activity) test, insulin secretion test, RNA isolation and cDNA synthesis assay, qPCR quantification assays, and statistical analyses were carried out in the present study. Alysine A exerted the following effects at non-cytotoxic doses: • Enhanced the glucose uptake, as much as the insulin in the C2C12, HepG2, and 3T3-L1 cells • Increased the glycogen content in the C2C12 and HepG2 liver cells, significantly higher than the insulin and metformin • Suppressed the alpha-glucosidase and the GLUT2 expression levels in the Caco-2 cells • Suppressed the SGLT1 and GLUT1-5 expression levels in the Caco-2 cells • Induced the insulin receptor substrate (IRS)1 and GLUT2 expression levels of the BTC6 pancreatic cells • Induced the insulin receptor (INSR), IRS2, phosphoinositide 3-kinase (PI3K), GLUT4, and protein kinase (PK) expression levels of the 3T3-L1 and C2C12 cells • Increased glucose transport through the Caco-2 cell layer • Did not influence insulin secretion in the pancreatic BTC6 cells Consequently, these data strongly emphasized the antidiabetic action of alysine A on the particularly critical model mechanisms that assume a part in glucose homeostasis, such as glucose uptake, utilization, and storage. Moreover, the expression level of the essential genes in glucose metabolism and insulin signaling was altered in a way that the results would be antihyperglycemic. A blend of in vitro and in situ tests affirmed the antihyperglycemic action of alysine A and its mechanism. Alysine A has exercised significant and positive results on the glucose homeostasis; thus, it is a natural and pleiotropic antidiabetic agent. Advanced in vivo studies are required to clarify the impact of this compound on glucose homeostasis completely | en_US |
dc.description.sponsorship | This work was supported by the Scientific and Technological Research Council of Turkey under TÜBİTAK, under Project No.: 114Z640, and Pamukkale University under Project No.: PAUBAP-2014FBE029. | en_US |
dc.language.iso | en | en_US |
dc.publisher | SCIENTIFIC TECHNICAL RESEARCH COUNCIL TURKEY-TUBITAK | en_US |
dc.rights | info:eu-repo/semantics/openAccess | en_US |
dc.subject | Alysine A, alysine B, antidiabetic, Teucrium alyssifolium, glucose homeostasis | en_US |
dc.title | Natural diterpenoid alysine A isolated from Teucrium alyssifolium exerts antidiabetic effect via enhanced glucose uptake and suppressed glucose absorption | en_US |
dc.type | Article | en_US |
dc.identifier.issue | 43 | en_US |
dc.identifier.startpage | 1350 | en_US |
dc.identifier.endpage | 1364 | en_US |
dc.authorid | 0000-0002-2306-6972 | - |
dc.identifier.doi | 10.3906/kim-1904-57 | - |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |
dc.identifier.scopus | 2-s2.0-85073800115 | en_US |
dc.identifier.trdizinid | 335965 | en_US |
dc.identifier.wos | WOS:000489111500010 | en_US |
dc.identifier.scopusquality | Q3 | - |
dc.owner | Pamukkale University | - |
item.languageiso639-1 | en | - |
item.openairetype | Article | - |
item.grantfulltext | open | - |
item.cerifentitytype | Publications | - |
item.fulltext | With Fulltext | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
crisitem.author.dept | 17.02. Biology | - |
crisitem.author.dept | 29. Denizli Health Services Vocational School of Higher Education | - |
crisitem.author.dept | 03.01. Organic Agriculture Management | - |
Appears in Collections: | Denizli Sağlık Hizmetleri Meslek Yüksekokulu Koleksiyonu Fen Fakültesi Koleksiyonu Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection TR Dizin İndeksli Yayınlar Koleksiyonu / TR Dizin Indexed Publications Collection Uygulamalı Bilimler Yüksekokulu Koleksiyonu WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection |
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My_Kim-43-5-10-1904-57.pdf | Makale Dosyası | 1.32 MB | Adobe PDF | View/Open |
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