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https://hdl.handle.net/11499/30271
Title: | Development of cardiac troponin-I biosensor based on boron nitride quantum dots including molecularly imprinted polymer | Authors: | Yola, M.L. Atar, Necip |
Keywords: | Boron nitride quantum dots Cardiac Troponin-I Molecular imprinted biosensor Plasma Biochemistry Biosensors Body fluids Boron nitride Cyclic voltammetry Diagnosis Drug products Electrochemical impedance spectroscopy Glass membrane electrodes Heart III-V semiconductors Mammals Nanocrystals Nitrides Plasmas Reusability Scanning electron microscopy Transmission electron microscopy Acute myocardial infarction Biochemical markers Bovine serum albumins Cardiac troponin I Characterization methods Glassy carbon electrodes Molecular imprinted biosensors Molecularly Imprinted Polymer Semiconductor quantum dots boron nitride quantum dot bovine serum albumin molecularly imprinted polymer myoglobin pyrrole quantum dot troponin I troponin T unclassified drug boron derivative boron nitride carbon polymer Article controlled study glassy carbon electrode human impedance spectroscopy limit of detection measurement repeatability protein blood level reproducibility scanning electron microscopy transmission electron microscopy X ray diffraction chemistry genetic procedures isolation and purification molecular imprinting Biosensing Techniques Boron Compounds Carbon Dielectric Spectroscopy Humans Limit of Detection Molecular Imprinting Myoglobin Polymers Quantum Dots Serum Albumin, Bovine Troponin I Troponin T |
Publisher: | Elsevier Ltd | Abstract: | The cardiac Troponin-I (cTnI) is one of the subunits of cardiac troponin complexes and a pivotal biochemical marker of acute myocardial infarction (AMI). Due to its myocardial specificity, cTnI is widely used for the diagnosis of AMI diseases. In this study, a novel imprinted biosensor approach based on boron nitride quantum dots (BNQDs) was presented for cTnI detection in plasma samples. Various characterization methods such as scanning electron microscope (SEM), transmission electron microscope (TEM), x-ray diffraction (XRD), cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) were used for all characterizations of nanomaterials. After the characterization analysis, cTnI imprinted electrode was developed in the presence of 100.0 mM pyrrole containing 25.0 mM cTnI. After that, the analytical studies of cTnI in plasma samples were performed by using cTnI imprinted biosensor. The results of the study have revealed that 0.01–5.00 ng mL -1 and 0.0005 ng mL -1 were found as the linearity range and the detection limit (LOD). Moreover, the selectivity of cTnI imprinted glassy carbon electrode (GCE) was investigated for plasma sample analysis in the presence of other nonspecific and specific proteins including cardiac myoglobin (MYG), bovine serum albumin (BSA) and cardiac troponin T (cTnT), respectively. Furthermore, the prepared biosensor was examined in terms of stability, repeatability, reproducibility and reusability. Finally, the imprinted biosensor was applied to the plasma samples having high recovery. © 2018 Elsevier B.V. | URI: | https://hdl.handle.net/11499/30271 https://doi.org/10.1016/j.bios.2018.11.016 |
ISSN: | 0956-5663 |
Appears in Collections: | Mühendislik Fakültesi Koleksiyonu PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection |
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