Evaluation of caffeic acid phenethyl ester administration in chronically stressed rats with experimental periodontitis

Abstract

Objectives: The aim of the present study was to investigate the therapeutic effects of systemic caffeic acid phenethyl ester treatment on oxidative stress and alveolar bone destruction in lipopolysaccharide (LPS)-induced periodontitis (EP) group in chronically stressed rats. Materials and Methods: Fourty male Sprague Dawley rats (EP-CS-CAPE) group were divided into four groups: 1) control group, 2) experimental periodontitis (EP), 3) EP and chronic stress (CS) group (EP-CS), and 4) EP-CS treated with CAPE (EP-CS-CAPE). To induce periodontitis, LPS was administered into the buccal gingiva of the test groups, and pure saline was administered for the interleukin (IL)-1? levels were control group. Two test groups were exposed to restraint stress and one group of these groups was treated with only a single dose of CAPE (10 mmol/kg). Likewise, saline was administered in the control, EP, and EP-CS groups. After 14 days, serum samples were collected from the heart, and all rats were sacrificed for analyses. Oxidative stress and interleukin (IL)-1? were investigated. The receptor activator of the nuclear factor kappa B ligand (RANKL) and alveolar bone loss were determined by immunohistochemical analysis. Results: The oxidative stress, alveolar bone loss, IL-1? and RANKL levels were found significantly higher in the EP-CS group compared with control and EP groups (p < 0.05). However, the administration of RANKL level CAPE significantly reduced oxidative stress and IL-1? in the EP-CS-CAPE group compared with the EP-CS group (p < 0.05). Also, CAPE treatment significantly reduced RANKL and alveolar bone loss in the EP-CS-CAPE group compared with the EP-CS group (p < 0.05). Conclusions: The present results indicated that CAPE may inhibit alveolar bone loss by modulating the immune response and inflammatory process. © 2019 Cumhuriyet University Faculty of Dentistry.

Amaç: Bu çalişmanin amaci sistemik kafeik asit fenetil ester (KAFE) uygulamasinin lipopolisakkarit (LPS) ile periodontitis oluşturulan kronik stresli siçanlarda oksidatif stres ve alveolar kemik kaybi üzerine terapötik etkisinin değerlendirilmesidir. Gereç ve Yöntemler: çalişmamizda 40 adet Sprague Dawley siçan kullanildi ve bu siçanlar dört gruba ayrildi: 1) kontrol grubu, 2) deneysel periodontitis (DP) grubu, 3) DP ve kronik stres (KS) grubu (DP-KS) ve 4) KAFE ile tedavi edilen DP-KS grup (DP-KS-KAFE). Periodontitis oluşturmak amaciyla LPS test gruplarinda bukkal dişetine uygulandi ve ayni yöntemle kontrol grubuna salin verildi. Bununla birlikte test gruplarindan ikisine kisitlama stresi uygulandi ve bu gruplardan biri de günlük tek doz KAFE ile tedavi edildi (10 mmol/kg). Benzer şekilde kontrol ve interlökin (IL)-1? seviyeleri incelendi. DP ve DP-KS gruplarina da salin uygulandi. 14 gün sonra, hayvanlarin kalbinden serum örnekleri toplandi ve bütün hayvanlar analiz için sakrifiye edildi. Oksidatif stres, interlökin (IL)-1? incelendi. Reseptör aktivatör nükleer faktör kappa B ligand (RANKL) ve alveolar kemik kaybi immünohistokimyasal olarak değerlendirildi. Bulgular: Oksidatif stres, alveoar kemik kaybi, IL-1? ve RANKL seviyeleri DP-KS grubunda kontrol ve DP gruplarina göre anlamli bir şekilde yüksek bulundu (p < 0,05). Ancak, KAFE uygulamasi oksidatif stres ve IL-1? seviyelerini DP-KS-KAFE grubunda DP-KS grubuna göre anlamli olarak azaltti (p < 0,05). Bununla birlikte yine KAFE tedavisi RANKL ve alveolar kemik kaybini DP-KS-KAFE grubunda DP-KS grubuna göre anlamli olarak azaltti (p < 0,05). Sonuçlar: Bu sonuçlar KAFE'nin immün cevabi ve enflamatuvar olaylari düzenleyerek alveolar kemik kaybini önleyebileceğini göstermiştir. © 2019 Cumhuriyet University Faculty of Dentistry.