Please use this identifier to cite or link to this item: https://hdl.handle.net/11499/36883
Title: Altered Expression of Long Non-coding RNAs in Peripheral Blood Mononuclear Cells of Patients with Alzheimer’s Disease
Authors: Kurt, S.
Tomatir, Ayşe Gaye
Tokgün, Pervin Elvan
Öncel, Çağatay
Keywords: Alzheimer’s
LncRNA
Microarray
PBMC
4 aminobutyric acid
long untranslated RNA
long untranslated RNA AL445989 1 2
long untranslated RNA CSTB 1
long untranslated RNA LINC01420
long untranslated RNA LOC401557
long untranslated RNA TTC39C AS1
messenger RNA
mitogen activated protein kinase
neurotrophin
phosphatidylinositol 3 kinase
protein kinase B
Ras protein
tumor necrosis factor
unclassified drug
adult
Alzheimer disease
Article
blood sampling
cholinergic synapse
clinical article
controlled study
dopaminergic system
down regulation
female
gene expression
glutamatergic synapse
hospital admission
human
human cell
male
microarray analysis
peripheral blood mononuclear cell
real time polymerase chain reaction
sequence analysis
signal transduction
upregulation
Publisher: Springer
Abstract: Long non-coding RNAs (lncRNAs) are involved in many neurological conditions, and neurodegenerative disorders including Alzheimer’s disease (AD) regulate gene expression at transcriptional, post-transcriptional, and epigenetic levels. However, the roles of lncRNAs in the pathogenesis of AD remain unclear. In this study, we aimed to determine the expression of lncRNAs and also mRNAs in AD which may alter expression and contribute to the pathogenesis of the disease. Peripheral blood samples were obtained from patients admitted to the Neurology Department of Pamukkale University Medical Faculty (23 patients with AD, 33 control groups). Total RNA obtained from peripheral blood mononuclear cells (PBMC) of subjects with probable AD (n = 4) and healthy control groups (n = 4) was examined to determine the altered expression of lncRNAs and mRNAs in AD were evaluated by microarray analysis. Five lncRNAs with the highest end-to-end fold change (fc ? 2.0, p < 0.05) were identified and confirmed by quantitative real-time polymerase chain reaction (qRT-PCR). In our study, the profiles of lncRNAs and mRNAs that may be associated with Alzheimer’s disease were determined. A total of 14 lncRNAs and 35 mRNAs were determined as upregulated, and 20 lncRNAs and 73 mRNAs determined as downregulated as a result of microarray analysis in patients with AD compared with control groups (fold change ? 2.0, p < 0.05). From lncRNAs, expression of lncRNA TTC39C-AS1, lnc-AL445989.1-2, LINC01420, lnc-CSTB-1, and LOC401557 was confirmed by qRT-PCR. When assessed by KEGG analysis of AD PBMC lncRNA and mRNA profiles, TNF signaling pathway, PI3K/AKT, Ras, and MAPK pathways; glutamatergic, dopaminergic, and cholinergic synapses; GABA, and neurotrophin signaling pathways are found to be significant. This is the first known study to investigate lncRNA profiles in AD PBMCs. We think that these results may open a door to the understanding of AD pathogenesis targeted by lncRNAs. © 2020, Springer Science+Business Media, LLC, part of Springer Nature.
URI: https://hdl.handle.net/11499/36883
https://doi.org/10.1007/s12035-020-02106-x
ISSN: 0893-7648
Appears in Collections:PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
Tıp Fakültesi Koleksiyonu
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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