Please use this identifier to cite or link to this item: https://hdl.handle.net/11499/36883
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dc.contributor.authorKurt, S.-
dc.contributor.authorTomatir, Ayşe Gaye-
dc.contributor.authorTokgün, Pervin Elvan-
dc.contributor.authorÖncel, Çağatay-
dc.date.accessioned2021-02-02T09:23:12Z
dc.date.available2021-02-02T09:23:12Z
dc.date.issued2020-
dc.identifier.issn0893-7648-
dc.identifier.urihttps://hdl.handle.net/11499/36883-
dc.identifier.urihttps://doi.org/10.1007/s12035-020-02106-x-
dc.description.abstractLong non-coding RNAs (lncRNAs) are involved in many neurological conditions, and neurodegenerative disorders including Alzheimer’s disease (AD) regulate gene expression at transcriptional, post-transcriptional, and epigenetic levels. However, the roles of lncRNAs in the pathogenesis of AD remain unclear. In this study, we aimed to determine the expression of lncRNAs and also mRNAs in AD which may alter expression and contribute to the pathogenesis of the disease. Peripheral blood samples were obtained from patients admitted to the Neurology Department of Pamukkale University Medical Faculty (23 patients with AD, 33 control groups). Total RNA obtained from peripheral blood mononuclear cells (PBMC) of subjects with probable AD (n = 4) and healthy control groups (n = 4) was examined to determine the altered expression of lncRNAs and mRNAs in AD were evaluated by microarray analysis. Five lncRNAs with the highest end-to-end fold change (fc ? 2.0, p < 0.05) were identified and confirmed by quantitative real-time polymerase chain reaction (qRT-PCR). In our study, the profiles of lncRNAs and mRNAs that may be associated with Alzheimer’s disease were determined. A total of 14 lncRNAs and 35 mRNAs were determined as upregulated, and 20 lncRNAs and 73 mRNAs determined as downregulated as a result of microarray analysis in patients with AD compared with control groups (fold change ? 2.0, p < 0.05). From lncRNAs, expression of lncRNA TTC39C-AS1, lnc-AL445989.1-2, LINC01420, lnc-CSTB-1, and LOC401557 was confirmed by qRT-PCR. When assessed by KEGG analysis of AD PBMC lncRNA and mRNA profiles, TNF signaling pathway, PI3K/AKT, Ras, and MAPK pathways; glutamatergic, dopaminergic, and cholinergic synapses; GABA, and neurotrophin signaling pathways are found to be significant. This is the first known study to investigate lncRNA profiles in AD PBMCs. We think that these results may open a door to the understanding of AD pathogenesis targeted by lncRNAs. © 2020, Springer Science+Business Media, LLC, part of Springer Nature.en_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofMolecular Neurobiologyen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAlzheimer’sen_US
dc.subjectLncRNAen_US
dc.subjectMicroarrayen_US
dc.subjectPBMCen_US
dc.subject4 aminobutyric aciden_US
dc.subjectlong untranslated RNAen_US
dc.subjectlong untranslated RNA AL445989 1 2en_US
dc.subjectlong untranslated RNA CSTB 1en_US
dc.subjectlong untranslated RNA LINC01420en_US
dc.subjectlong untranslated RNA LOC401557en_US
dc.subjectlong untranslated RNA TTC39C AS1en_US
dc.subjectmessenger RNAen_US
dc.subjectmitogen activated protein kinaseen_US
dc.subjectneurotrophinen_US
dc.subjectphosphatidylinositol 3 kinaseen_US
dc.subjectprotein kinase Ben_US
dc.subjectRas proteinen_US
dc.subjecttumor necrosis factoren_US
dc.subjectunclassified drugen_US
dc.subjectadulten_US
dc.subjectAlzheimer diseaseen_US
dc.subjectArticleen_US
dc.subjectblood samplingen_US
dc.subjectcholinergic synapseen_US
dc.subjectclinical articleen_US
dc.subjectcontrolled studyen_US
dc.subjectdopaminergic systemen_US
dc.subjectdown regulationen_US
dc.subjectfemaleen_US
dc.subjectgene expressionen_US
dc.subjectglutamatergic synapseen_US
dc.subjecthospital admissionen_US
dc.subjecthumanen_US
dc.subjecthuman cellen_US
dc.subjectmaleen_US
dc.subjectmicroarray analysisen_US
dc.subjectperipheral blood mononuclear cellen_US
dc.subjectreal time polymerase chain reactionen_US
dc.subjectsequence analysisen_US
dc.subjectsignal transductionen_US
dc.subjectupregulationen_US
dc.titleAltered Expression of Long Non-coding RNAs in Peripheral Blood Mononuclear Cells of Patients with Alzheimer’s Diseaseen_US
dc.typeArticleen_US
dc.identifier.volume57en_US
dc.identifier.issue12en_US
dc.identifier.startpage5352
dc.identifier.startpage5352en_US
dc.identifier.endpage5361en_US
dc.identifier.doi10.1007/s12035-020-02106-x-
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.identifier.pmid32885358en_US
dc.identifier.scopus2-s2.0-85090128125en_US
dc.identifier.wosWOS:000565831800004en_US
dc.identifier.scopusqualityQ1-
dc.ownerPamukkale University-
item.cerifentitytypePublications-
item.languageiso639-1en-
item.openairetypeArticle-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.fulltextNo Fulltext-
item.grantfulltextnone-
crisitem.author.dept14.03. Basic Medical Sciences-
crisitem.author.dept14.02. Internal Medicine-
Appears in Collections:PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
Tıp Fakültesi Koleksiyonu
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection
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