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https://hdl.handle.net/11499/36883
Title: | Altered Expression of Long Non-coding RNAs in Peripheral Blood Mononuclear Cells of Patients with Alzheimer’s Disease | Authors: | Kurt, S. Tomatir, Ayşe Gaye Tokgün, Pervin Elvan Öncel, Çağatay |
Keywords: | Alzheimer’s LncRNA Microarray PBMC 4 aminobutyric acid long untranslated RNA long untranslated RNA AL445989 1 2 long untranslated RNA CSTB 1 long untranslated RNA LINC01420 long untranslated RNA LOC401557 long untranslated RNA TTC39C AS1 messenger RNA mitogen activated protein kinase neurotrophin phosphatidylinositol 3 kinase protein kinase B Ras protein tumor necrosis factor unclassified drug adult Alzheimer disease Article blood sampling cholinergic synapse clinical article controlled study dopaminergic system down regulation female gene expression glutamatergic synapse hospital admission human human cell male microarray analysis peripheral blood mononuclear cell real time polymerase chain reaction sequence analysis signal transduction upregulation |
Publisher: | Springer | Abstract: | Long non-coding RNAs (lncRNAs) are involved in many neurological conditions, and neurodegenerative disorders including Alzheimer’s disease (AD) regulate gene expression at transcriptional, post-transcriptional, and epigenetic levels. However, the roles of lncRNAs in the pathogenesis of AD remain unclear. In this study, we aimed to determine the expression of lncRNAs and also mRNAs in AD which may alter expression and contribute to the pathogenesis of the disease. Peripheral blood samples were obtained from patients admitted to the Neurology Department of Pamukkale University Medical Faculty (23 patients with AD, 33 control groups). Total RNA obtained from peripheral blood mononuclear cells (PBMC) of subjects with probable AD (n = 4) and healthy control groups (n = 4) was examined to determine the altered expression of lncRNAs and mRNAs in AD were evaluated by microarray analysis. Five lncRNAs with the highest end-to-end fold change (fc ? 2.0, p < 0.05) were identified and confirmed by quantitative real-time polymerase chain reaction (qRT-PCR). In our study, the profiles of lncRNAs and mRNAs that may be associated with Alzheimer’s disease were determined. A total of 14 lncRNAs and 35 mRNAs were determined as upregulated, and 20 lncRNAs and 73 mRNAs determined as downregulated as a result of microarray analysis in patients with AD compared with control groups (fold change ? 2.0, p < 0.05). From lncRNAs, expression of lncRNA TTC39C-AS1, lnc-AL445989.1-2, LINC01420, lnc-CSTB-1, and LOC401557 was confirmed by qRT-PCR. When assessed by KEGG analysis of AD PBMC lncRNA and mRNA profiles, TNF signaling pathway, PI3K/AKT, Ras, and MAPK pathways; glutamatergic, dopaminergic, and cholinergic synapses; GABA, and neurotrophin signaling pathways are found to be significant. This is the first known study to investigate lncRNA profiles in AD PBMCs. We think that these results may open a door to the understanding of AD pathogenesis targeted by lncRNAs. © 2020, Springer Science+Business Media, LLC, part of Springer Nature. | URI: | https://hdl.handle.net/11499/36883 https://doi.org/10.1007/s12035-020-02106-x |
ISSN: | 0893-7648 |
Appears in Collections: | PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection Tıp Fakültesi Koleksiyonu WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection |
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