An effective non-enzymatic biosensor platform based on copper nanoparticles decorated by sputtering on CVD graphene

Abstract

It has become inevitable to design non-enzymatic biosensors to eliminate the drawbacks of enzymatic biosensors prepared using enzymes which are expensive and without long-term stability. For this purpose, a single layer graphene film was prepared by chemical vapor deposition method on Cu foil and transferred to the FTO glass slide. After that copper nanoparticles (CuNP) were decorated by the inert-gas condensation method based on DC magnetron sputtering on it. The prepared CuNP decorated graphene film was characterized and used as a non-enzymatic sensor platform for the detection of glucose. The sensor platform exhibited a fast response time of less than 4 s and the sensitivity of 430.52 µA mM-1 cm-2 with linear concentration range (0.01–1.0 mM) having detection limit 7.2 µM. Electrochemical investigations indicate that the sensor platform which is decorated CuNP graphene film possess an excellent performance toward glucose. Prepared biosensors platform could be used and applied in the field of new drug discovery, biomedical, clinical diagnosis and forensic science to miniaturize of detection instrument and reduce detection sample and period.