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https://hdl.handle.net/11499/4025
Title: | Effects of arginine and trehalose on postthawed bovine sperm quality | Authors: | Ozturk, Caner Gungor, Şükrü Ataman, Mehmet Bozkurt Bucak, Mustafa Numan Başpınar, Nuri İli, Pınar Inanc, Muhammed Enes |
Keywords: | Cell Biology, Sperm Cryopreservation | Publisher: | Akademia Kiado Rt | Abstract: | The present study was conducted to examine the protective role of arginine and trehalose on post-thaw bull sperm and oxidative stress parameters. Five ejaculates for each bull were used in the study. Each ejaculate, split into three equal aliquots and diluted at 37 degrees C with base extenders containing 2 mM arginine, 25 mM trehalose and no antioxidant (control) was cooled to 5 degrees C and then frozen. Frozen straws were thawed in a water bath for evaluation. Supplementation of the semen extender with arginine decreased the percentages of post-thawed subjective motility (29 +/- 8.21%), CASA motility (12.2 +/- 5.69%) and progressive motility (3.52 +/- 2.13%), compared with the controls (43 +/- 2.73%, 55.4 +/- 6.78% and 33.48 +/- 4.14%, respectively, P < 0.05). Supplementation of the semen extender with trehalose produced a higher mitochondrial activity and sperm viability (36.3 +/- 3.99% and 44.1 +/- 2.18%) compared with the control (13 +/- 8.15 and 31.7 +/- 3.94%, respectively, P < 0.05). It was established that trehalose (95.1%) and arginine (92.8%) protect DNA integrity compared to the control (90.4%) (P < 0.05). Trehalose supplementation in semen extenders provided great benefit in terms of viability, mitochondrial activity, and intact sperm DNA on frozen-thawed bull sperm. | URI: | https://hdl.handle.net/11499/4025 | ISSN: | 0236-6290 |
Appears in Collections: | Denizli Sağlık Hizmetleri Meslek Yüksekokulu Koleksiyonu PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection |
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Effects of Arginine and Trehalose on Post Thawed Bovine Sperm Quality.pdf | 171.6 kB | Adobe PDF | View/Open |
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