Please use this identifier to cite or link to this item:
https://hdl.handle.net/11499/4300
Title: | Immunohistochemical detection and distribution of cornifin ? and vitamin D receptor expression in vaginal epithelium treated with vitamin D 3 | Other Titles: | Vitamin D3 verilen sıçanların vajen epitelinde kornifin alfa ve vitamin D reseptör ekspresyonun immünohistokimyasal olarak belirlenmesi | Authors: | Yildirim, Başak Nazan. Abban, Gülçin. Rota, Simin. Jetten, A. |
Keywords: | Cornifin ? Immunohistochemistry Vagina VDR Vitamin D colecalciferol cornifin cornifin alpha unclassified drug vitamin D receptor animal cell animal experiment article cell differentiation cell growth cell proliferation controlled study drug effect female histopathology immunohistochemistry nonhuman rat vagina epithelium |
Abstract: | Objective: In growth and differentation of keratinizing stratified squamous epithelium, 1.25-dihydroxyvitamin D3 has an important role. Cornifin ? as a marker of vaginal epithelial differentiation distribution in vagina epithelium has not been studied. We examined distribution of VDR and cornifin ? in vagina epithelium in ovariectomized and treated vitamin D rats. Materials and Methods: Bilateral ovariectomies were performed in 20 mature non-pregnant Wistar female rats. All the animals were divided into 2 groups; control group I untreated and group II i.m. 0.025 µg 1.25-dihydroxyvitamin D3 injected for three days of a week for 2 weeks. In 15 days after the last injection, vaginas were removed and processed for immunohistochemistry. Results: Recorded epithelial differentiation, 1.25-dihydroxyvitamin D 3 receptor and cornifin ? expression in vaginal epithelium of control and vitamin D3 treated rats. In treated animals, vaginal epithelium changed into highly-stratified keratinizing layers. Cornifin ? and 1.25-dihydroxyvitamin D3 receptor as a marker of squamous differentiation is present in ovariectomized rats treated with 1.25-dihydroxyvitamin D3. In contrast, cornifin ? and 1.25-dihydroxyvitamin D3 receptor were absent in all layers of vaginal epithelium in control group. Discussion: The results indicate that 1.25-dihydroxyvitamin D3 induced proliferation of vaginal epithelium which was consistent with the cornifin ? expression. We demonstrated for the first time that 1.25-dihydroxyvitamin D3 up-regulated 1.25-dihydroxyvitamin D3 receptor expression in vaginal epithelium. | URI: | https://hdl.handle.net/11499/4300 | ISSN: | 1309-0399 |
Appears in Collections: | Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection Tıp Fakültesi Koleksiyonu WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection |
Show full item record
CORE Recommender
SCOPUSTM
Citations
1
checked on Aug 17, 2024
Page view(s)
46
checked on Aug 24, 2024
Google ScholarTM
Check
Items in GCRIS Repository are protected by copyright, with all rights reserved, unless otherwise indicated.