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https://hdl.handle.net/11499/4300
Title: | Immunohistochemical detection and distribution of cornifin ? and vitamin D receptor expression in vaginal epithelium treated with vitamin D 3 | Other Titles: | Vitamin D3 verilen sıçanların vajen epitelinde kornifin alfa ve vitamin D reseptör ekspresyonun immünohistokimyasal olarak belirlenmesi | Authors: | Yildirim, Başak Nazan. Abban, Gülçin. Rota, Simin. Jetten, A. |
Keywords: | Cornifin ? Immunohistochemistry Vagina VDR Vitamin D colecalciferol cornifin cornifin alpha unclassified drug vitamin D receptor animal cell animal experiment article cell differentiation cell growth cell proliferation controlled study drug effect female histopathology immunohistochemistry nonhuman rat vagina epithelium |
Abstract: | Objective: In growth and differentation of keratinizing stratified squamous epithelium, 1.25-dihydroxyvitamin D3 has an important role. Cornifin ? as a marker of vaginal epithelial differentiation distribution in vagina epithelium has not been studied. We examined distribution of VDR and cornifin ? in vagina epithelium in ovariectomized and treated vitamin D rats. Materials and Methods: Bilateral ovariectomies were performed in 20 mature non-pregnant Wistar female rats. All the animals were divided into 2 groups; control group I untreated and group II i.m. 0.025 µg 1.25-dihydroxyvitamin D3 injected for three days of a week for 2 weeks. In 15 days after the last injection, vaginas were removed and processed for immunohistochemistry. Results: Recorded epithelial differentiation, 1.25-dihydroxyvitamin D 3 receptor and cornifin ? expression in vaginal epithelium of control and vitamin D3 treated rats. In treated animals, vaginal epithelium changed into highly-stratified keratinizing layers. Cornifin ? and 1.25-dihydroxyvitamin D3 receptor as a marker of squamous differentiation is present in ovariectomized rats treated with 1.25-dihydroxyvitamin D3. In contrast, cornifin ? and 1.25-dihydroxyvitamin D3 receptor were absent in all layers of vaginal epithelium in control group. Discussion: The results indicate that 1.25-dihydroxyvitamin D3 induced proliferation of vaginal epithelium which was consistent with the cornifin ? expression. We demonstrated for the first time that 1.25-dihydroxyvitamin D3 up-regulated 1.25-dihydroxyvitamin D3 receptor expression in vaginal epithelium. | URI: | https://hdl.handle.net/11499/4300 | ISSN: | 1309-0399 |
Appears in Collections: | Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection Tıp Fakültesi Koleksiyonu WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection |
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