In Vitro Antioxidant Property of Convolvulus aucheri and Its NO Inhibitory Potential in LPSactivated NSCLC Cells

Abstract

Latterly, scientists have turned into progressively curious about therapeutic herbs. A lotof Convolvulus taxa display different biologic activities. In this study, we aimed to evaluate nitricoxide (NO) inhibitive effect of Convolvulus aucheri in LPS-induced non-small cell lung cancer(NSCLC) cells, such as HCC78 and H1975 and determine its antioxidant capacity. While Griess assaywas applied to determine the nitrite level as an index of NO production, ?-carotene/linoleic acid testwas used for the determining antioxidant effect. Cytotoxic activity of C. aucheri extract was detectedby using CellTiter-Glo assay. The extract induced a bigger cytotoxic activity on H1975 cells than theHCC78 cells and decreased NO production in concentration-dependent manner. The highest inhibitorypotential against NO formation was observed to be 113.06 µM nitrite at a concentration of 40 ?g/mLin HCC78 cells. As for the antioxidant activity, C. aucheri showed the highest antioxidant activity of70.89%. These data bring to mind that C. aucheri may be a useful source for exploration newanticancer compounds.