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https://hdl.handle.net/11499/4518
Title: | Surface plasmon resonance-based molecular detection of Hb S [ß6(A3)Glu›Val, GAG›GTG] at the gene level | Authors: | Atalay, Erol Ömer Üstel, E. Yildiz, S. Atalay, Ayfer |
Keywords: | Abnormal hemoglobin (Hb) Hb S Mutation detection Surface plasmon resonance (SPR) adenine glutamic acid guanine hemoglobin S hemoglobin variant thymine valine article codon controlled study cost diagnostic value genetic analysis globin gene hemoglobinopathy human human genetics medical examination mutational analysis screening test surface plasmon resonance Genetic Counseling Globins Hemoglobin, Sickle Humans Point Mutation Premarital Examinations Surface Plasmon Resonance |
Abstract: | The surface plasmon resonance (SPR) approach, being a relatively novel biophysical method, is used to detect many different targets by biomolecular interaction. The SPR system uses optical and evanescent wave phenomenon. This approach does not need any labels, such as enzymes or isotopes, and the monitored interactions are in real time. In DNA-DNA interaction, the SPR approach is Tm-independent. Here we report our preliminary results for the molecular detection of the Hb S (G A G ›G T G) mutation at codon 6 of the human ß-globin gene. Our preliminary results show that the SPR approach could be applied as an inexpensive and fast routine test system for the molecular diagnosis of abnormal hemoglobins (Hbs), especially in premarital screening programs. Copyright © Taylor & Francis Group, LLC. | URI: | https://hdl.handle.net/11499/4518 https://doi.org/10.1080/03630260600755807 |
ISSN: | 0363-0269 |
Appears in Collections: | PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection Tıp Fakültesi Koleksiyonu WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection |
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