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Title: | Evaluation of Viral and Bacterial Pathogens in the Central Nervous System Infections with Multiplex PCR | Authors: | Oner S.Z. Kaleli I. Demir M. Mete E. Caliskan A. |
Keywords: | Bacteria culture Bacterial multiplex PCR Viral multiplex PCR Adenoviridae adult Article bacterial growth bacterium bacterium identification central nervous system infection cerebrospinal fluid analysis Cytomegalovirus descriptive research Epstein Barr virus evaluation study female Herpes simplex virus Herpes simplex virus 2 human Human alphaherpesvirus 1 Human herpesvirus 7 Human parvovirus B19 major clinical study male multiplex polymerase chain reaction Neisseria meningitidis nonhuman Streptococcus pneumoniae Turkey (republic) Varicella zoster virus virus young adult central nervous system infection Epstein Barr virus infection genetics turkey (bird) Bacteria Central Nervous System Infections Epstein-Barr Virus Infections Herpesvirus 4, Human Humans Turkey |
Publisher: | College of Physicians and Surgeons Pakistan | Abstract: | Objective: To evaluate the bacterial and viral causes of central nervous system (CNS) infection by multiplex PCR. Study Design: Descriptive study. Place and Duration of Study: Department of Medical Microbiology, Pamukkale University Faculty of Medicine, Turkey, from March 2016 to December 2021. Methodology: Cerebrospinal fluid (CSF) samples of patients prediagnosed with CNS infection were included in the study. Viral pathogens were detected with the Multiplex real-time PCR panel (FTD Neuro9, Fast Track Diagnostics, Luxembourg) and bacterial pathogens with the multiplex real-time PCR panel (FTD Bacterial Meningitis, Fast Track Diagnostics, Luxembourg). The identification of bacteria growing in samples was done by conventional methods and with the Phoenix™ (Becton Dickinson Diagnostics, USA) automated system. Results: CSF samples of 440 patients were evaluated using multiplex PCR panel. The viral factors included adenovirus (14.2%), human herpes virus 7 (1.5%), varicella zoster virus (1.3%), herpes simplex virus 1 (1.3%), cytomegalovirus (1.3%), Epstein-Barr virus (0.8%), human herpes virus (0.8%), herpes simplex virus 2 (0.3%), varicella zoster virus (0.3%), and parvovirus B19 (0.3%); and bacterial factors included Streptococcus pneumoniae (7.0%) and Neisseria meningitidis (0.9%). The bacterial growth was detected in the CSF culture was 4.9%. Among the growing bacteria, there were six different types that were not found on the multiplex PCR panel. Conclusion: The use of a comprehensive bacterial multiplex PCR panel containing common pathogens will be more effective in pathogen detection. Care should be taken, especially when interpreting the viral Multiplex PCR. © 2022 College of Physicians and Surgeons Pakistan. All rights reserved. | URI: | https://doi.org/10.29271/jcpsp.2022.12.1605 https://hdl.handle.net/11499/47715 |
ISSN: | 1022-386X |
Appears in Collections: | PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection Tıp Fakültesi Koleksiyonu WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection |
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