Protective effect of Toki-shakuyaku-san on amyloid ß25-35- induced neuronal damage in cultured rat cortical neurons

Abstract

Amyloid ß protein (Aß) is the major component of senile plaques, the pathological hallmark of the neurodegeneration associated with Alzheimer's disease (AD). This study investigated the effect of Toki-shakuyaku-san (TSS), a traditional medicine, on Aß25-35-induced neuronal death and lipid peroxidation assessed by measuring lactate dehydrogenase (LDH) and malondialdehyde (MDA), respectively. Aß25-35 at 10 µM induced neuronal damage and increased the LDH and MDA. TSS at concentrations of 100 and 300 µg/mL significantly reduced the Aß25-35-induced neuronal death and the lipid peroxidation. These results suggest that TSS has a protective effect against Aß25-35-induced neuronal damage. TSS may be beneficial for the treatment of AD. Copyright © 2005 John Wiley & Sons, Ltd.