A successful encapsulation technique for the production of synthetic seeds for lilium candidum l. grown naturally in Turkey

Abstract

Lilium candidum L. (white lily) is in danger of extinction due to the destruction of its natural habitat in Turkey and the removal of its tubers from nature for export. For this reason, it is very important to carry out biotechnological studies with L. candidum, which is an important medicinal and ornamental plant. In vitro techniques can provide the opportunity to preserve plant genetic resources in different forms and different genetic features (synthetic seed). With these techniques, it is possible to reproduce plant species rapidly in danger of extinction and transfer them to their natural environments with in vitro and ex situ conservation studies. In this study, we aimed to establish a suitable protocol for the production of synthetic seeds in L. candidum. Accordingly, regeneration was achieved by using the stem bulbils of the plant. An effective encapsulation technique has been created for the production of synthetic seeds in L. candidum, obtained from its calli by regeneration. The calculated callus development and embryogenesis rates of encapsulated calli were higher than single ones, 92.5%-88.6%, and 90.4%-77.1% respectively. Our protocol turned out to be successful for synthetic seed production as well as large-scale in vitro clonal propagation of L. candidum.