Please use this identifier to cite or link to this item: https://hdl.handle.net/11499/5444
Title: Role of dopaminergic system in core part of nucleus accumbens in hyperlocomotion and rearing induced by MK-801 in rats: A behavioral and in vivo microdialysis study
Authors: Hatip-Al-Khatib, İzzettin
Bölükbaşı, Funda Fatma
Mishima, K.
Egashira, N.
Iwasaki, K.
Fujiwara, M.
Keywords: Core nucleus accumbens (rat)
Microdialysis
MK-801
Motor activity
3,4 dihydroxyphenylacetic acid
amine
desipramine
dizocilpine
dopamine
oxidopamine
serotonin
animal experiment
animal tissue
article
behavior
brain injury
cell level
comparative study
concentration (parameters)
controlled study
dopaminergic system
dose response
drug effect
extracellular space
high performance liquid chromatography
hyperactivity
in vivo study
male
microdialysis
motor activity
nonhuman
nucleus accumbens
rat
rearing
3,4-Dihydroxyphenylacetic Acid
Animals
Chromatography, High Pressure Liquid
Dizocilpine Maleate
Dopamine
Electrochemistry
Excitatory Amino Acid Antagonists
Extracellular Space
Male
Motor Activity
Nucleus Accumbens
Oxidopamine
Rats
Rats, Wistar
Serotonin
Stereotaxic Techniques
Sympathectomy, Chemical
Sympatholytics
Abstract: We investigated modification of the MK-801 effect on motor activity and extracellular amines concentration by 6-hydroxydopamine (6-OHDA)-induced lesion of core nucleus accumbens (cACC) of rats. In vivo microdialysis-HPLC showed that the concentrations (fmol/µl) of dopamine (DA), dihydroxyphenylacetic acid (DOPAC) and serotonin were 0.738±0.135, 155.34±41.01 and 0.334±0.024, respectively, in the cACC of intact rats. The DOPAC/DA ratio was 264.24±94.01. Unilateral lesion of the cACC with 6-OHDA (8 µg/µl) substantially reduced DA (-93%) and DOPAC (-97%) in desipramine (30 mg/kg, i.p.)-pretreated rats (6-OHDA+DMI rats) as compared to the 65% reduction rate of both amines in saline-pretreated rats (6-OHDA+saline rats). Moreover, DOPAC was reduced by 72% in 6-OHDA+DMI rats. MK-801 increased DOPAC (426-467%) and DOPAC/DA ratio (180-230%) in intact rats. On the other hand, MK-801 increased DA by 154% and 505% in 6-OHDA+saline and 6-OHDA+DMI rats, respectively. 6-OHDA reduced the effect of MK-801 on DOPAC and DOPAC/DA ratio. In the behevioral studies, MK-801 (0.01 - 0.3 mg/kg, i.p.) increased locomotor activity and rearing of intact rats. Bilateral 6-OHDA+DMI mesion of the cACC caused greater reduction in the effect of MK-801 (0.1 mg/kg) than that of the shell nucleus accumbens. These results suggest that increased extracellular DOPAC concentration (but not DA) and DOPAC/DA ratio in the cACC plays an important role in MK-801-hyperactivity.
URI: https://hdl.handle.net/11499/5444
https://doi.org/10.1254/jjp.87.277
ISSN: 0021-5198
Appears in Collections:PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
Tıp Fakültesi Koleksiyonu
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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