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https://hdl.handle.net/11499/5444
Title: | Role of dopaminergic system in core part of nucleus accumbens in hyperlocomotion and rearing induced by MK-801 in rats: A behavioral and in vivo microdialysis study | Authors: | Hatip-Al-Khatib, İzzettin Bölükbaşı, Funda Fatma Mishima, K. Egashira, N. Iwasaki, K. Fujiwara, M. |
Keywords: | Core nucleus accumbens (rat) Microdialysis MK-801 Motor activity 3,4 dihydroxyphenylacetic acid amine desipramine dizocilpine dopamine oxidopamine serotonin animal experiment animal tissue article behavior brain injury cell level comparative study concentration (parameters) controlled study dopaminergic system dose response drug effect extracellular space high performance liquid chromatography hyperactivity in vivo study male microdialysis motor activity nonhuman nucleus accumbens rat rearing 3,4-Dihydroxyphenylacetic Acid Animals Chromatography, High Pressure Liquid Dizocilpine Maleate Dopamine Electrochemistry Excitatory Amino Acid Antagonists Extracellular Space Male Motor Activity Nucleus Accumbens Oxidopamine Rats Rats, Wistar Serotonin Stereotaxic Techniques Sympathectomy, Chemical Sympatholytics |
Abstract: | We investigated modification of the MK-801 effect on motor activity and extracellular amines concentration by 6-hydroxydopamine (6-OHDA)-induced lesion of core nucleus accumbens (cACC) of rats. In vivo microdialysis-HPLC showed that the concentrations (fmol/µl) of dopamine (DA), dihydroxyphenylacetic acid (DOPAC) and serotonin were 0.738±0.135, 155.34±41.01 and 0.334±0.024, respectively, in the cACC of intact rats. The DOPAC/DA ratio was 264.24±94.01. Unilateral lesion of the cACC with 6-OHDA (8 µg/µl) substantially reduced DA (-93%) and DOPAC (-97%) in desipramine (30 mg/kg, i.p.)-pretreated rats (6-OHDA+DMI rats) as compared to the 65% reduction rate of both amines in saline-pretreated rats (6-OHDA+saline rats). Moreover, DOPAC was reduced by 72% in 6-OHDA+DMI rats. MK-801 increased DOPAC (426-467%) and DOPAC/DA ratio (180-230%) in intact rats. On the other hand, MK-801 increased DA by 154% and 505% in 6-OHDA+saline and 6-OHDA+DMI rats, respectively. 6-OHDA reduced the effect of MK-801 on DOPAC and DOPAC/DA ratio. In the behevioral studies, MK-801 (0.01 - 0.3 mg/kg, i.p.) increased locomotor activity and rearing of intact rats. Bilateral 6-OHDA+DMI mesion of the cACC caused greater reduction in the effect of MK-801 (0.1 mg/kg) than that of the shell nucleus accumbens. These results suggest that increased extracellular DOPAC concentration (but not DA) and DOPAC/DA ratio in the cACC plays an important role in MK-801-hyperactivity. | URI: | https://hdl.handle.net/11499/5444 https://doi.org/10.1254/jjp.87.277 |
ISSN: | 0021-5198 |
Appears in Collections: | PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection Tıp Fakültesi Koleksiyonu WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection |
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