Effect of gonadotropins on blood, bone marrow and spleen in cyclophosphamide exposed rats

Abstract

Cyclophosphamide (CTX) is an effective chemotherapeutic agent. Gonadotropins are molecules with various actions. Here, we investigated the effects of gonadotropins on the peripheral blood, bone marrow and spleen in rats administered with CTX. Three groups were formed: Control (C) group with no process; Sham (S) group: Physiological saline was applied; CTX group: A single dose of 200 mg/kg and 8 mg/kg CTX was administered for the next 14 days. All rats were superovulated with 150-300 IU/kg pregnant mare serum gonadotropin. Human chorionic gonadotropin @150-300 IU/kg was given, and complete blood counts, bone marrow smears, and spleen sections were examined; and also the expression of WNT-1, WNT-4, and 0-catenin was analyzed. Although the hemoglobin and platelet value in the CTX group was lowest, it was still within the normal reference ranges. The C and S groups had significantly higher white blood cell values (p=0.017). In terms of number of megakaryocytes, Myeloid/ Erythroid ratio, lymphoid cell ratios, no significant differences were found in bone marrow aspiration smears. The CTX group had significantly higher 0-catenin expression in the red pulp than the other groups (p=0.0001). The CTX group had the highest WNT-4 expression and very intense expression of WNT-1 in the white pulp. Our results indicate that the gonadotropins, promising in treatment, have favourable effects on toxicity of CTX.