Please use this identifier to cite or link to this item: https://hdl.handle.net/11499/56254
Title: Effect of gonadotropins on blood, bone marrow and spleen in cyclophosphamide exposed rats
Authors: Ünver Koluman, Başak
Çil, Nazlı
Mete, Gülçin Abban
Keywords: Cytotoxic chemotherapy
Drug toxicity
Human Chorionic-Gonadotropin
Hormone
Pathway
Mice
Publisher: Natl Inst Science Communication-Niscair
Abstract: Cyclophosphamide (CTX) is an effective chemotherapeutic agent. Gonadotropins are molecules with various actions. Here, we investigated the effects of gonadotropins on the peripheral blood, bone marrow and spleen in rats administered with CTX. Three groups were formed: Control (C) group with no process; Sham (S) group: Physiological saline was applied; CTX group: A single dose of 200 mg/kg and 8 mg/kg CTX was administered for the next 14 days. All rats were superovulated with 150-300 IU/kg pregnant mare serum gonadotropin. Human chorionic gonadotropin @150-300 IU/kg was given, and complete blood counts, bone marrow smears, and spleen sections were examined; and also the expression of WNT-1, WNT-4, and 0-catenin was analyzed. Although the hemoglobin and platelet value in the CTX group was lowest, it was still within the normal reference ranges. The C and S groups had significantly higher white blood cell values (p=0.017). In terms of number of megakaryocytes, Myeloid/ Erythroid ratio, lymphoid cell ratios, no significant differences were found in bone marrow aspiration smears. The CTX group had significantly higher 0-catenin expression in the red pulp than the other groups (p=0.0001). The CTX group had the highest WNT-4 expression and very intense expression of WNT-1 in the white pulp. Our results indicate that the gonadotropins, promising in treatment, have favourable effects on toxicity of CTX.
URI: https://doi.org/10.56042/ijeb.v61i11.1764
https://hdl.handle.net/11499/56254
ISSN: 0019-5189
0975-1009
Appears in Collections:Tıp Fakültesi Koleksiyonu
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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