Please use this identifier to cite or link to this item: https://hdl.handle.net/11499/56868
Title: Biosourced polymeric cryogels for future biomedical applications with remarkable antimicrobial activities and tribological properties
Authors: Gürel, C.M.
Bozbeyoğlu, N.N.
Yardımcı, B.K.
Şarkaya, K.
Mutlu, D.
Akıncıoğlu, S.ı.
Doğan, N.M.
Arslan, Şevki
Allı, Abdülkadir
Keywords: Antibiofilm activity
Antifungal activity
Antimicrobial hydrogels
Biocompatibility
Cryogels
Hydroxylated fatty acids
Polymicrobial biofilm
Bacteria
Cartilage
Cell culture
Cell proliferation
Hydrogels
Hydroxylation
Linoleic acid
Medical applications
Scaffolds (biology)
Tribology
Yeast
Anti-microbial activity
Antibiofilm activity
Antibiofilms
Antifungal activities
Antimicrobial hydrogel
Biomedical applications
Cryogels
Hydroxylated fatty acids
Polymicrobial biofilm
Tribological properties
Biocompatibility
Publisher: Elsevier Ltd
Abstract: Cryogels, known as a subclass of hydrogels, are promising biomaterials to use in various biotechnological fields. In recent years, applications of antimicrobial hydrogels with improved antimicrobial activities, high biocompatibility, and physicochemical stability have attracted attention as an alternative to using antimicrobial drugs against microbial interactions that may threaten human health, which may even result in death. In this paper, we investigated in detail the biological activities and tribological performances of the previously characterized 2-hydroxyethyl methacrylate (HEMA)-based amphiphilic cryogels (PHEMA-PLinaOH) (HC series) that contain hydroxylated polymeric linoleic acid (PLinaOH) as biosource. The biocompatibilities of these cryogels were examined against human embriyonic kidney (HEK293) cell line with MTT assay and acridine orange/ethidium bromide (AO/EB) dual staining. The antimicrobial activities of the materials were extensively investigated against Staphylococcus aureus ATCC 29213 and Pseudomonas aeruginosa PA01 besides four different strains of the yeast Saccharomyces cerevisiae BY4741 by using biofilms eradication, antibiofilm activity and colony forming unit assays. Additionally, the possible morphological changes in microbial cells were evaluated by taking FESEM images. The tribological performances of the cryogels were evaluated in terms of their applicability for future biomedical applications such as artificial articular cartilage or tissue scaffold. Our results showed that while the cryogels did not show significant inhibition on HEK293 cell viability and intensive live cell population was observed after AO/EB staining, they exerted remarkable antimicrobial activities against all studied bacterial and fungal strains. The morphological deformations including the decrease in EPS density and formation of holes were recorded for bacteria and yeast cells with FESEM images, respectively. Finally, it was determined that the increase in the fatty acid ratio contributes positively to tribological properties of the cryogels. All the results indicate that these polymeric cryogels might be considered potential biomaterials for future tissue-engineering studies. © 2024 Elsevier Ltd
URI: https://doi.org/10.1016/j.mtcomm.2024.108387
https://hdl.handle.net/11499/56868
ISSN: 2352-4928
Appears in Collections:Fen Fakültesi Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
Tavas Meslek Yüksekokulu Koleksiyonu
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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