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https://hdl.handle.net/11499/5916
Title: | Quercetin-induced apoptosis involves increased hTERT enzyme activity of leukemic cells | Authors: | Avci, C.B. Yilmaz, S. Dogan, Z.O. Saydam, G. Dodurga, Yavuz Ekiz, H.A. Kartal, M. |
Keywords: | Apoptosis hTERT Leukemia Quercetin Telomerase acridine orange ethidium bromide quercetin telomerase reverse transcriptase trypan blue acute lymphoblastic leukemia antineoplastic activity apoptosis article cancer inhibition cell proliferation cell viability chronic myeloid leukemia concentration response controlled study drug cytotoxicity drug effect enzyme activity enzyme assay enzyme repression fluorescence microscopy human human cell leukemia cell lymphatic leukemia priority journal promyelocytic leukemia Antineoplastic Agents Antioxidants Cell Line, Tumor Cell Survival Dose-Response Relationship, Drug Enzyme Activation Gene Expression Regulation, Leukemic HL-60 Cells Humans K562 Cells RNA, Messenger |
Abstract: | We aimed to examine the growth suppressive effects of quercetin on acute promyelocytic and lymphoblastic leukemia and chronic myeloid leukemia, and to find out whether the growth suppression is related to the blocking of telomerase enzyme activity. Cytotoxic effects of quercetin were shown by trypan blue analyses. Apoptotic effects of quercetin were examined by acridine orange and ethidium bromide staining by fluorescence microscopy. The effects of quercetin on telomerase enzyme activity were shown by hTERT Quantification Kit. Our results demonstrated that quercetin has antiproliferative and apoptotic effects on T-cell acute lymphoblastic leukemia (ALL), acute promyelocytic leukemia, and chronic myeloid leukemia (CML) cells. We also showed for the first time by this study that quercetin suppresses the activity of telomerase in ALL and CML cells. The results of this study show the importance of quercetin for its therapeutic potential in treatment of leukemias. © W. S. Maney & Son Ltd 2011. | URI: | https://hdl.handle.net/11499/5916 https://doi.org/10.1179/102453311X13085644680104 |
ISSN: | 1024-5332 |
Appears in Collections: | PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection Tıp Fakültesi Koleksiyonu WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection |
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