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https://hdl.handle.net/11499/8053
Title: | Raffinose and hypotaurine improve the post-thawed Merino ram sperm parameters | Authors: | Bucak, M.N. Keskin, Nazan Taşpinar, M. Çoyan, Kenan Başpinar, N. Cenariu, M.C. Bilgili, A. |
Keywords: | Additives Comet test Flourescent staining parameters Motility Ram sperm TUNEL-positive cells DNA hypotaurine raffinose acrosome animal cell article cell protection cell viability controlled study cryopreservation DNA damage freeze thawing male mitochondrion nonhuman priority journal sperm spermatozoon motility thawing Animals Cell Membrane Cryopreservation DNA Damage Male Mitochondria Raffinose Semen Preservation Sheep Sperm Motility Spermatozoa Taurine |
Abstract: | The aim of this study was to determine the effects of raffinose and hypotaurine on sperm parameters after the freeze-thawing of Merino ram sperm. Totally 40 ejaculates of five Merino ram were used in the study. Semen samples, which were diluted with a Tris-based extender containing 10. mM raffinose, 5. mM hypotaurine, 5. mM raffinose +2.5. mM hypotaurine (H + R) and no antioxidant (control), were cooled to 5 °C and frozen in 0.25. ml French straws and stored in liquid nitrogen. Frozen straws were then thawed individually at 37 °C for 25. s in a water bath for evaluation. The addition of raffinose led to higher percentages of subjective and CASA motilities (47.5 ± 12.2%, 46.3 ± 13.6%) compared to controls (38.8 ± 13.8%, 30.5 ± 11.7%, P< 0.05). For the CASA progressive motility, 5. mM raffinose (20.12 ± 8.82%) had increasing effect in comparison to control (10 ± 7.94%, P< 0.05) following the freeze-thawing process. Raffinose and hypotaurine led to higher viability (40.8 ± 4.68%, 40.8 ± 4.7%), high sperm mitochondrial activity (29.5 ± 5.4%, 27.3 ± 4.9%) and acrosome integrity (50.8 ± 8.1, 50.7 ± 4.4) percentages, compared to control groups (31.5 ± 3.5%, 9.5 ± 8.2%, 42.8 ± 7.3%, P< 0.05). H + R group only led to high sperm mitochondrial activity when compared to control group. In the comet test, raffinose and hypotaurine resulted in lower sperm with damaged DNA (6.2% and 3.9%) than that of control (9.1%), reducing the DNA damage. For TUNEL assay, The TUNEL-positive cell was distinguished by distinct nuclear staining. Raffinose and H + R groups resulted in lower sperm with TUNEL-positive cell (1.5 ± 1.2% and 2.1 ± 0.9%) than that of control (4.9 ± 2.5%) (P< 0.05). In conclusion, findings of this study showed that raffinose and hypotaurine supplementation in semen extenders provided a better protection of sperm parameters against cryopreservation injury, in comparison to the control groups. © 2013. | URI: | https://hdl.handle.net/11499/8053 https://doi.org/10.1016/j.cryobiol.2013.04.007 |
ISSN: | 0011-2240 |
Appears in Collections: | PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection Tıp Fakültesi Koleksiyonu WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection |
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