Please use this identifier to cite or link to this item: https://hdl.handle.net/11499/8053
Title: Raffinose and hypotaurine improve the post-thawed Merino ram sperm parameters
Authors: Bucak, M.N.
Keskin, Nazan
Taşpinar, M.
Çoyan, Kenan
Başpinar, N.
Cenariu, M.C.
Bilgili, A.
Keywords: Additives
Comet test
Flourescent staining parameters
Motility
Ram sperm
TUNEL-positive cells
DNA
hypotaurine
raffinose
acrosome
animal cell
article
cell protection
cell viability
controlled study
cryopreservation
DNA damage
freeze thawing
male
mitochondrion
nonhuman
priority journal
sperm
spermatozoon motility
thawing
Animals
Cell Membrane
Cryopreservation
DNA Damage
Male
Mitochondria
Raffinose
Semen Preservation
Sheep
Sperm Motility
Spermatozoa
Taurine
Abstract: The aim of this study was to determine the effects of raffinose and hypotaurine on sperm parameters after the freeze-thawing of Merino ram sperm. Totally 40 ejaculates of five Merino ram were used in the study. Semen samples, which were diluted with a Tris-based extender containing 10. mM raffinose, 5. mM hypotaurine, 5. mM raffinose +2.5. mM hypotaurine (H + R) and no antioxidant (control), were cooled to 5 °C and frozen in 0.25. ml French straws and stored in liquid nitrogen. Frozen straws were then thawed individually at 37 °C for 25. s in a water bath for evaluation. The addition of raffinose led to higher percentages of subjective and CASA motilities (47.5 ± 12.2%, 46.3 ± 13.6%) compared to controls (38.8 ± 13.8%, 30.5 ± 11.7%, P< 0.05). For the CASA progressive motility, 5. mM raffinose (20.12 ± 8.82%) had increasing effect in comparison to control (10 ± 7.94%, P< 0.05) following the freeze-thawing process. Raffinose and hypotaurine led to higher viability (40.8 ± 4.68%, 40.8 ± 4.7%), high sperm mitochondrial activity (29.5 ± 5.4%, 27.3 ± 4.9%) and acrosome integrity (50.8 ± 8.1, 50.7 ± 4.4) percentages, compared to control groups (31.5 ± 3.5%, 9.5 ± 8.2%, 42.8 ± 7.3%, P< 0.05). H + R group only led to high sperm mitochondrial activity when compared to control group. In the comet test, raffinose and hypotaurine resulted in lower sperm with damaged DNA (6.2% and 3.9%) than that of control (9.1%), reducing the DNA damage. For TUNEL assay, The TUNEL-positive cell was distinguished by distinct nuclear staining. Raffinose and H + R groups resulted in lower sperm with TUNEL-positive cell (1.5 ± 1.2% and 2.1 ± 0.9%) than that of control (4.9 ± 2.5%) (P< 0.05). In conclusion, findings of this study showed that raffinose and hypotaurine supplementation in semen extenders provided a better protection of sperm parameters against cryopreservation injury, in comparison to the control groups. © 2013.
URI: https://hdl.handle.net/11499/8053
https://doi.org/10.1016/j.cryobiol.2013.04.007
ISSN: 0011-2240
Appears in Collections:PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
Tıp Fakültesi Koleksiyonu
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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