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https://hdl.handle.net/11499/9755
Title: | Association between herbivore stress and glutathione S-transferase expression in Pinus brutia Ten | Authors: | Semiz, Aslı Çelik-Turgut, Gurbet Semiz, Gürkan Özgün, Özden Şen, Alaattin |
Keywords: | Glutathione S-transferase Pinus brutia Stress physiology Thaumetopoea wilkinsoni glutathione transferase messenger RNA plant protein Article controlled study enzyme activity herbivore nonhuman orchard pine protein expression reverse transcription polymerase chain reaction stress Thaumetopoea animal gene expression regulation genetics herbivory host parasite interaction moth parasitology physiology plant disease Animals Gene Expression Regulation, Plant Glutathione Transferase Herbivory Host-Parasite Interactions Moths Pinus Plant Diseases Plant Proteins |
Publisher: | Cellular and Molecular Biology Association | Abstract: | Plants have developed mechanisms to defend themselves against many factors including biotic stress such as herbivores and pathogens. Glutathione S-transferase (GST) is a glutathione-dependent detoxifying enzyme and plays critical roles in stress tolerance and detoxification metabolism in plants. Pinus brutia Ten. is a prominent native forest tree species in Turkey, due to both its economic and ecological assets. One of the problems faced by P. brutia afforestation sites is the attacks by pine processionary moth (Thaumetopoea wilkinsoni Tams.). In this study, we investigated the changes in activity and mRNA expression of GST in pine samples taken from both resistant and susceptible clones against T. wilkinsoni over a nine month period in a clonal seed orchard. It was found that the average cytosolic GST activities of trees in March and July were significantly higher than the values obtained in November. November was considered to be the control since trees were not under stress yet. In addition, RT-PCR results clearly showed that levels of GST transcripts in March and July samples were significantly higher as compared to the level seen in November. These findings strongly suggest that GST activity from P. brutia would be a valuable marker for exposure to herbivory stress. © 2016 by the C.M.B. Association. | URI: | https://hdl.handle.net/11499/9755 https://doi.org/10.14715/cmb/2016.62.3.15 |
ISSN: | 0145-5680 |
Appears in Collections: | Fen-Edebiyat Fakültesi Koleksiyonu PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection Teknoloji Fakültesi Koleksiyonu Uygulamalı Bilimler Yüksekokulu Koleksiyonu WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection |
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