Please use this identifier to cite or link to this item: https://hdl.handle.net/11499/9760
Title: Vitamin-D receptor (VDR) gene polymorphisms (TaqI, FokI) in Turkish patients with Hashimoto’s thyroiditis: Relationship to the levels of Vit-D and Cytokines
Authors: Güleryüz, Bedia
Akın, Hatice Fulya
Ata,Melek Tunç
Dalyanoğlu,Mukaddes Mergen
Turgut, Sebahat
Keywords: Hashimoto’s thyroiditis
IFN-?
IL-2
IL-4
IL-5
TNF-? levels
Vitamin D
Vitamin D receptor gene polymorphism
alkaline phosphatase
calcifediol
calcium
cytokine
gamma interferon
glucose
insulin
interleukin 2
interleukin 4
interleukin 5
parathyroid hormone
phosphate
thyrotropin
thyroxine
tumor necrosis factor
vitamin D receptor
adult
Article
blood level
case control study
controlled study
DNA polymorphism
echography
female
FokI gene
gene
genetic analysis
Hashimoto disease
human
major clinical study
male
polymerase chain reaction
prevalence
restriction fragment length polymorphism
TaqI gene
Publisher: Bentham Science Publishers B.V.
Abstract: Background: Hashimoto’s thyroiditis (HT) is a common autoimmune disease. Vitamin D is an important regulator of immune system. It has been shown in several studies that vitamin D prevents the development of lots of autoimmune diseases. There are some studies that prove vitamin D receptor (VDR) gene polymorphism increases the risk of Hashimoto’s thyroiditis. In this study, we aimed to investigate the association between HT and level of 25(OH)D3, IL-2, IL-4, IL-5, TNF-? and IFN-? and VDR FokI and TaqI gene polymorphism. Moreover, to find out whether low levels of vitamin D affect HT pathogenesis over inflammatory parameters. Methods: We performed a case-control study that included 136 cases with HT (49 euthyroid, 49 subclinical hypothyroid, 38 hypothyroid patients) and 50 healthy control. Serum levels of 25(OH)D3, glucose, insulin, parathyroid hormone, calcium, phosphorus, alkaline phosphatase were measured and IL-4, IL-5, TNF-?, IFN-? analysis were performed with ELISA kits in all 186 subjects. Genetic analysis for VDR FokI and TaqI gene polymorphisms were done by RFLP in all subjects. Results: Mean serum 25(OH)D levels were 14.88±8.23 ng/ml in patient with HT and 15.52±1.34 ng/ml in healthy controls. There were no statically significant differences between the groups in terms of vitamin D levels (P=0.977). Prevalence of vitamin D insufficiency in HT cases was significantly higher than controls (p=0.02). Although serum IL-2, IL-4, TNF-? and IFN-? were significantly higher in HT patients, there were no significant differences regarding IL-5 levels. Significant differences were observed between the groups regarding the genotype of TaqI but no differences regarding FokI genotype. Conclusion: Vitamin D insufficiency is associated with HT. There is a relationship between VDR TaqI gene polymorphism and HT. Although vitamin D levels are low in both patient and control group, detection of high level of inflammatory parameters in HT group makes us think that low level of vitamin D does not affect HT pathogenesis over these parameters. © 2016 Bentham Science Publishers.
URI: https://hdl.handle.net/11499/9760
https://doi.org/10.2174/1871530316666160728092613
ISSN: 1871-5303
Appears in Collections:Denizli Sağlık Hizmetleri Meslek Yüksekokulu Koleksiyonu
PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
Tıp Fakültesi Koleksiyonu
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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