Genotoxicological assessment of nebuloside-A a triterpenoid saponin compound on whole blood DNA

Abstract

We investigated the genotoxic effect of nebuloside-A on whole blood DNA by using alkaline Single Cell Gel Electrophoresis Comet assay. Saponins are a widespread class of bioactive compounds produced by many plant species. Nebuloside A was isolated from babys-breath (Gypsophila arrostii) by high performance liquid chromatography method. Mass spectra obtained by electrospray ionization-time of flight-mass spectrometry in a negative ion mode to reduce the protonation. Complete nuclear magnetic resonance spectroscopy assignments of nebuloside A was achieved by using 2D-nuclear magnetic resonance spectroscopy techniques, such as double-quantum filtered correlation spectroscopy, heteronuclear single quanthum correlation, heteronuclear multiple-bond correlation, and total correlation spectroscopy. DNA damage was measured in total arbitrary units by visual scoring of comets in different concentrations of nebuloside A and H2O2 treatments. We found that significant dose dependent relationship between nebuloside A and its genotoxic effect. © 2015 Taylor & Francis Group, LLC.