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https://hdl.handle.net/11499/36890
Title: | In vitro antimicrobial, antioxidant, cytotoxic activities, and wound healing potential of Thymbra capitata ethanolic extract | Other Titles: | Thymbra capitata etanolik ekstraktının in vitro antimikrobiyal, antioksidan, sitotoksik aktiviteleri ve yara iyileşme potansiyeli | Authors: | Hepokur, C. Misir, S. Tunc, T. Tutar, U. Çiçek, Mehmet Hepokur, Ali İhsan |
Keywords: | Antimicrobial Antioxidant Cytotoxic activity Thymbra capitata Wound healing |
Publisher: | De Gruyter Open Ltd | Abstract: | Objectives: In this study, we aimed to detect the chemical compounds of Thymbra capitata ethanolic extract (TC-EtOH) as well as to evaluated its antimicrobial, antioxidant, cytotoxic activities, and in vivo wound healing effects. Methods: The chemical composition of TC-EtOH was analyzed by Gas chromatography-mass spectrometry (GC-MS). Antioxidant and antimicrobial properties were determined with 2,2-diphenyl-1-picrylhydrazyl (DPPH), disc diffusion test and broth micro-dilution (minimal inhibitory concentration [MIC]) methods, respectively. Cytotoxic activity was tested on MG63 (human osteosarcoma) and MCF-7 (human breast carcinoma) cells by 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium- 5-carboxanilide (XTT) assay. Tumor necrosis factor alpha (TNF-?) protein levels were determined by ELISA. Results: The major components of TC-EtOH were tetratria contane (14.92%), camphor (12.50%), and terpineol (10.77%). TC-EtOH showed powerful antimicrobial activity in C. Tropicalis (0.03 mg/mL). The IC50 values of the TC-EtOH of the DPPH were determined 21.5 µg/mL. The IC50 values were calculated 37.28 and 44.40 µg/mL on the MG63 and MCF-7 cell lines, respectively. It was observed that the wounds treated with TC-EtOH showed a faster healing. Conclusions: According to results, T. capitata species are thought to be natural antioxidants and a novel pharmaceutical compound for the pharmaceutical industry. © 2020 De Gruyter. All rights reserved. | URI: | https://hdl.handle.net/11499/36890 https://doi.org/10.1515/tjb-2019-0470 |
ISSN: | 0250-4685 |
Appears in Collections: | Fen-Edebiyat Fakültesi Koleksiyonu Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection TR Dizin İndeksli Yayınlar Koleksiyonu / TR Dizin Indexed Publications Collection WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection |
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